SOST, an LNGFR target, inhibits the osteogenic differentiation of rat ectomesenchymal stem cells.
Cell Prolif
; 51(2): e12412, 2018 Apr.
Article
en En
| MEDLINE
| ID: mdl-29226516
ABSTRACT
OBJECTIVES:
The aim of this study was to investigate whether sclerostin (SOST) regulates the osteogenic differentiation of rat ectomesenchymal stem cells (EMSCs) and whether SOST and low-affinity nerve growth factor receptor (LNGFR) regulate the osteogenic differentiation of EMSCs. MATERIALS ANDMETHODS:
EMSCs were isolated from embryonic facial processes from an embryonic 12.5-day (E12.5d) pregnant Sprague-Dawley rat. LNGFR+ EMSCs and LNGFR- EMSCs were obtained by fluorescence-activated cell sorting and were subsequently induced to undergo osteogenic differentiation in vitro. SOST/LNGFR small-interfering RNAs and SOST/LNGFR overexpression plasmids were used to transfect EMSCs.RESULTS:
LNGFR+ EMSCs displayed a higher osteogenic capacity and lower SOST levels compared with LNGFR- EMSCs. SOST silencing enhanced the osteogenic differentiation of LNGFR- EMSCs, while SOST overexpression attenuated the osteogenic differentiation of LNGFR+ EMSCs. Moreover, LNGFR was present upstream of SOST and strengthened the osteogenic differentiation of EMSCs by decreasing SOST.CONCLUSIONS:
SOST alleviated the osteogenic differentiation of EMSCs, and LNGFR enhanced the osteogenic differentiation of EMSCs by decreasing SOST, suggesting that the LNGFR/SOST pathway may be a novel target for promoting dental tissue regeneration and engineering.Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Osteogénesis
/
Diferenciación Celular
/
Receptores de Factores de Crecimiento
/
Proteínas Morfogenéticas Óseas
/
Células Madre Embrionarias
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Células Madre Mesenquimatosas
/
Proteínas del Tejido Nervioso
Límite:
Animals
Idioma:
En
Revista:
Cell Prolif
Año:
2018
Tipo del documento:
Article
País de afiliación:
China