Essential role of amino acid position 71 in substrate preference by meso-diaminopimelate dehydrogenase from Symbiobacterium thermophilum IAM14863.

Zhang, Yanan; Ma, Qinyuan; Dong, Miaomiao; Zhang, Xianhai; Chen, Yichu; Gao, Xiuzhen; Song, Yuanda

Zhang, Yanan; Ma, Qinyuan; Dong, Miaomiao; Zhang, Xianhai; Chen, Yichu; Gao, Xiuzhen; Song, Yuanda.

Afiliación

Zhang Y; School of Life Science, Shandong University of Technology, Zibo 255000, People's Republic of China.
Ma Q; Key Laboratory of Industrial Fermentation Microbiology (Tianjin University of Science & Technology), Ministry of Education, Tianjin 300457, People's Republic of China; Tianjin Key Lab of Industrial Microbiology, College of Biotechnology, Tianjin University of Science and Technology, Tianjin 3004
Dong M; School of Life Science, Shandong University of Technology, Zibo 255000, People's Republic of China.
Zhang X; School of Life Science, Shandong University of Technology, Zibo 255000, People's Republic of China.
Chen Y; School of Life Science, Shandong University of Technology, Zibo 255000, People's Republic of China.
Gao X; School of Life Science, Shandong University of Technology, Zibo 255000, People's Republic of China. Electronic address: gaoxz@sdut.edu.cn.
Song Y; School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo 255000, People's Republic of China.

Enzyme Microb Technol ; 111: 57-62, 2018 Apr.

Article en En | MEDLINE | ID: mdl-29421037

Asunto(s)

Aminoácido Oxidorreductasas/química; Aminoácido Oxidorreductasas/metabolismo; Proteínas Bacterianas/química; Proteínas Bacterianas/metabolismo; Clostridiales/enzimología; Aminoácido Oxidorreductasas/genética; Sustitución de Aminoácidos; Proteínas Bacterianas/genética; Sitios de Unión/genética; Biocatálisis; Clostridiales/genética; Ácido Diaminopimélico/química; Ácido Diaminopimélico/metabolismo; Cinética; Modelos Moleculares; Mutagénesis Sitio-Dirigida; NADP/metabolismo; Conformación Proteica; Estereoisomerismo; Especificidad por Sustrato

Palabras clave

Cation-π interaction; Non-active site; Reductive amination; Substrate preference; meso-Diaminopimelate dehydrogenase

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas Bacterianas / Clostridiales / Aminoácido Oxidorreductasas Tipo de estudio: Prognostic_studies Idioma: En Revista: Enzyme Microb Technol Año: 2018 Tipo del documento: Article Pais de publicación: Estados Unidos

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