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MicroRNA-409 inhibits the proliferative ability of cervical carcinoma cells by regulating AKT.
Li, B; Wu, N; Zhang, X-J; Wei, Z-L; Shang, L-X.
Afiliación
  • Li B; Department of Obstetrics and Gynecology, Anhui Medical University Affiliated First Hospital, Hefei, China. Weizhaolian_1@126.com.
Eur Rev Med Pharmacol Sci ; 22(4): 936-942, 2018 02.
Article en En | MEDLINE | ID: mdl-29509241
OBJECTIVE: To observe the microRNA-409-3p expression in cervical carcinoma and its effect on the growth and proliferative ability of cervical carcinoma cells. PATIENTS AND METHODS: The microRNA-409-3p level in 62 cases of cervical carcinoma and 38 cases of normal cervical tissue was detected by qRT-PCR. The association between the microRNA-409-3p level and clinicopathological features of cervical carcinoma was investigated. Knockdown and overexpressed microRNA-409-3p in cervical carcinoma cells, HeLa and SiHa, were used to detect the cell cycle and the activity of cervical carcinoma cells. Subsequently, potentially target genes of microRNA-409-3p were predicted by bioinformatics website. Western Blot and luciferase assay were used to confirm their correlation. RESULTS: We observed a significant lower microRNA-409-3p level in cervical carcinoma tissues than that in normal cervical tissues. Significant correlation was found between the microRNA-409-3p level in patients with cervical carcinoma and the overall survival rate, tumor size and TNM stage (p<0.05), but correlations with age, pathological type and lymph node metastasis were not found (p>0.05). After silencing the expression of microRNA-409-3p in cervical carcinoma cells, the proliferative ability of cervical carcinoma cells was greatly promoted. In addition, microRNA-409-3p was targeting on protein kinase B (AKT) and had a negative correlation with AKT expression. CONCLUSIONS: The microRNA-409-3p level was lower in cervical carcinoma tissues, and was significantly affected the overall survival, tumor size and TNM staging in clinical patients. Low expression of microRNA-409-3p could promote the proliferative ability of cervical carcinoma cells through the target gene AKT.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias del Cuello Uterino / MicroARNs / Proliferación Celular / Proteínas Proto-Oncogénicas c-akt Límite: Female / Humans / Middle aged Idioma: En Revista: Eur Rev Med Pharmacol Sci Asunto de la revista: FARMACOLOGIA / TOXICOLOGIA Año: 2018 Tipo del documento: Article País de afiliación: China Pais de publicación: Italia

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias del Cuello Uterino / MicroARNs / Proliferación Celular / Proteínas Proto-Oncogénicas c-akt Límite: Female / Humans / Middle aged Idioma: En Revista: Eur Rev Med Pharmacol Sci Asunto de la revista: FARMACOLOGIA / TOXICOLOGIA Año: 2018 Tipo del documento: Article País de afiliación: China Pais de publicación: Italia