A Method to Assess Fc-mediated Effector Functions Induced by Influenza Hemagglutinin Specific Antibodies.
J Vis Exp
; (132)2018 02 23.
Article
en En
| MEDLINE
| ID: mdl-29553549
ABSTRACT
Antibodies play a crucial role in coupling the innate and adaptive immune responses against viral pathogens through their antigen binding domains and Fc-regions. Here, we describe how to measure the activation of Fc effector functions by monoclonal antibodies targeting the influenza virus hemagglutinin with the use of a genetically engineered Jurkat cell line expressing an activating type 1 Fc-FcγR. Using this method, the contribution of specific Fc-FcγR interactions conferred by immunoglobulins can be determined using an in vitro assay.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Receptores Fc
/
Receptores de IgG
/
Gripe Humana
/
Hemaglutininas
Límite:
Humans
Idioma:
En
Revista:
J Vis Exp
Año:
2018
Tipo del documento:
Article