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Experimental listeriosis: A study of purinergic and cholinergic inflammatory pathway.
Jaguezeski, Antonise M; da Silva, Aleksandro S; Gomes, Teane M A; Bottari, Nathieli B; Lopes, Thalisson F; Cechin, Renan A; Morsch, Vera M; Schetinger, Maria R C; Giongo, Janice L; de A Vaucher, Rodrigo.
Afiliación
  • Jaguezeski AM; Department of Molecular Biology and Toxicological Biochemistry, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil; Department of Animal Science, Universidade do Estado de Santa Catarina, Chapecó, Santa Catarina, Brazil.
  • da Silva AS; Department of Molecular Biology and Toxicological Biochemistry, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil; Department of Animal Science, Universidade do Estado de Santa Catarina, Chapecó, Santa Catarina, Brazil. Electronic address: aleksandro.silva@udesc.br.
  • Gomes TMA; Laboratory of Veterinary Pathology, Instituto Federal Catarinense - IFC, Concórdia, Santa Catarina, Brazil.
  • Bottari NB; Department of Molecular Biology and Toxicological Biochemistry, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.
  • Lopes TF; Department of Molecular Biology and Toxicological Biochemistry, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.
  • Cechin RA; Laboratory of Veterinary Pathology, Instituto Federal Catarinense - IFC, Concórdia, Santa Catarina, Brazil.
  • Morsch VM; Department of Molecular Biology and Toxicological Biochemistry, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.
  • Schetinger MRC; Department of Molecular Biology and Toxicological Biochemistry, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.
  • Giongo JL; Pharmacy Laboratory, Faculdade Anhanguera, Pelotas, RS, Brazil.
  • de A Vaucher R; Department of Biochemistry, Universidade Federal de Pelotas, RS, Brazil.
Vet Microbiol ; 241: 108528, 2020 Feb.
Article en En | MEDLINE | ID: mdl-31882365
The cholinergic, purinergic and oxidative stress systems were related to nervous system damage in some pathologies, as well as being involved in pro-inflammatory and anti-inflammatory pathways. The objective was to investigate changes in purinergic, cholinergic systems and oxidative stress related to the neuropathology of listeriosis. Gerbils were used as experimental models. The animals were divided in two groups: control and infected. The animals were orally infected with 5 × 108 CFU/animal of the pathogenic strain of Listeria monocytogenes. Collected of material was 6 and 12th days post-infection (PI). Infected animals showed moderate mixed inflammatory infiltrates in the liver. The spleen and brain was used for PCR analyses, confirming infection by L. monocytogenes. Increase in number of total leukocytes because of an increase in lymphocytes in infected (P < 0.001). ATP and ADP hydrolysis by NTPDase was lower at 6 and 12th days PI in infected animals than in the control group. ADA (adenosine deaminase) activity was higher on the 6th day PI (P < 0.05) and decreased on the 12th day PI (P < 0.05) in infected animals. AChE (acetylcholinesterase) activity did not differ between groups on the 6th day PI; however, activity decreased in infected group on the 12th day PI (P < 0.05). On the 12th day PI, an increase of oxygen-reactive species levels and lower catalase and superoxide dismutase activities in the infected group was observed, characterizing a situation of cerebral oxidative stress. The inflammatory and oxidative mechanisms are present in listeriosis in asymptomatic animals, and that ectonucleotidases and cholinesterase's are involved in immunomodulation.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Listeriosis / Listeria monocytogenes Límite: Animals Idioma: En Revista: Vet Microbiol Año: 2020 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Países Bajos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Listeriosis / Listeria monocytogenes Límite: Animals Idioma: En Revista: Vet Microbiol Año: 2020 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Países Bajos