Tailoring translational strength using Kozak sequence variants improves bispecific antibody assembly and reduces product-related impurities in CHO cells.

Blanco, Noelia; Williams, Ambrose J; Tang, Danming; Zhan, Dejin; Misaghi, Shahram; Kelley, Robert F; Simmons, Laura C

Blanco, Noelia; Williams, Ambrose J; Tang, Danming; Zhan, Dejin; Misaghi, Shahram; Kelley, Robert F; Simmons, Laura C.

Afiliación

Blanco N; Departments of Cell Culture, Genentech, Inc., 1 DNA Way, South San Francisco, California.
Williams AJ; Departments of Purification Development, Genentech, Inc., 1 DNA Way, South San Francisco, California.
Tang D; Departments of Cell Culture, Genentech, Inc., 1 DNA Way, South San Francisco, California.
Zhan D; Departments of Cell Culture, Genentech, Inc., 1 DNA Way, South San Francisco, California.
Misaghi S; Departments of Cell Culture, Genentech, Inc., 1 DNA Way, South San Francisco, California.
Kelley RF; Departments of Drug Delivery, Genentech, Inc., 1 DNA Way, South San Francisco, California.
Simmons LC; Departments of Cell Culture, Genentech, Inc., 1 DNA Way, South San Francisco, California.

Biotechnol Bioeng ; 117(7): 1946-1960, 2020 07.

Article en En | MEDLINE | ID: mdl-32246763

Asunto(s)

Anticuerpos Biespecíficos/genética; Animales; Células CHO; Cricetulus; Biblioteca de Genes; Biosíntesis de Proteínas; Proteínas Recombinantes de Fusión/genética; Transfección

Palabras clave

Kozak sequence; bispecific antibody production; chain ratios; mammalian cell culture; translational strength

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Anticuerpos Biespecíficos Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Biotechnol Bioeng Año: 2020 Tipo del documento: Article Pais de publicación: Estados Unidos

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