[The Relationship between HIF1α and WTAP Expression Level in t(8;21) Acute Myeloid Leukemia].
Zhongguo Shi Yan Xue Ye Xue Za Zhi
; 29(5): 1424-1428, 2021 Oct.
Article
en Zh
| MEDLINE
| ID: mdl-34627420
ABSTRACT
OBJECTIVE:
To investigate the relationship between hypoxia inducible factor 1 (HIF1α) and Wilms' tumor 1îassociating protein (WTAP) expression level in t(8;21) acute myeloid leukemia cells.METHODS:
The t(8;21) acute myeloid leukemia cell lines, including SKNO-1 and Kasumi-1 were treated by Echinomycin for 24 h, RT-qPCR and Western blot were used to detect the expression levels of WTAP mRNA and the protein. The CoCl 2 was used to induce the hypoxia of the cells for 24 h, the expression levels of HIF1α, WTAP protein were detected by Western blot.RESULTS:
The expression level of WTAP mRNA and the protein in the echinomycin treated group was significantly lower than those in the control group (P<0.01). The expression level of WTAP protein in the CoCl2 treated group was significantly higher than those in the control group (P<0.05).CONCLUSION:
The inhibition of HIF1-α could down-regulates the expression of WTAP, while the up-regulation of HIF1α could up-regulates the expression of WTAP, which shows that there is a positive correlation of HIF1α and WTAP expression. This result suggesting that HIF1α may be involves in the expression regulation of WTAP gene.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Leucemia Mieloide Aguda
/
Proteínas de Ciclo Celular
Límite:
Humans
Idioma:
Zh
Revista:
Zhongguo Shi Yan Xue Ye Xue Za Zhi
Asunto de la revista:
HEMATOLOGIA
Año:
2021
Tipo del documento:
Article
País de afiliación:
China