A competitive ligand-binding assay to detect neutralizing antibodies to a bispecific drug using a multiplex Meso Scale Discovery platform.
Bioanalysis
; 13(22): 1659-1669, 2021 Nov.
Article
en En
| MEDLINE
| ID: mdl-34743610
ABSTRACT
Background:
Monitoring appearance of neutralizing antibodies (NAbs) to multidomain large molecule drugs is a challenging task. Materials &methods:
Here, we report development of a competitive ligand-binding assay for detection of NAbs to a bispecific candidate drug using a multiplex Meso Scale Discovery platform, which allows for detection of NAbs to both drug arms in the same sample.Results:
The assay has sensitivity better than 250 ng/ml and is tolerant to the presence of drug at concentration >600 µg/ml and to the level of soluble target(s) >400 ng/ml.Conclusion:
Our data suggest that multiplex approach can be successfully used for development of NAb assays in competitive ligand-binding assay format.Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Inmunoensayo
/
Anticuerpos Neutralizantes
/
Ligandos
Límite:
Female
/
Humans
/
Male
Idioma:
En
Revista:
Bioanalysis
Año:
2021
Tipo del documento:
Article
País de afiliación:
Estados Unidos