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Oxytetracycline Degrading Potential of Lysinibacillus sp. Strain 3+I Isolated from Poultry Manure.
Sudha, Suruttaiyan; Parthasarathi, Nivethitha; Prabha, Duraisamy; Velmurugan, Palanivel; Sivakumar, Subpiramaniyam; Anitha, Vijayakumar; Shrestha, Anupama; Chinnathambi, Arunachalam; Alharb, Sulaiman Ali; Lakshmanaperumalsamy, Perumalsamy.
Afiliación
  • Sudha S; Department of Environmental Sciences, Bharathiar University, Coimbatore, Tamil Nadu, India.
  • Parthasarathi N; Department of Environmental Sciences, Bharathiar University, Coimbatore, Tamil Nadu, India.
  • Prabha D; Department of Environmental Sciences, Bharathiar University, Coimbatore, Tamil Nadu, India.
  • Velmurugan P; Centre for Materials Engineering and Regenerative Medicine, Bharath Institute of Higher Education and Research, Selaiyur, Chennai 600073, Tamil Nadu, India.
  • Sivakumar S; Department of Bioenvironmental Energy, College of Natural Resources and Life Science, Pusan National University, Miryang-Si, Gyeongsangnam-do 50463, Republic of Korea.
  • Anitha V; Department of Environmental Sciences, Bharathiar University, Coimbatore, Tamil Nadu, India.
  • Shrestha A; Department of Plant Protection, Himalayan College of Agricultural Sciences and Technology, P.O. Box 44600 Kalanki, Kathmandu, Nepal.
  • Chinnathambi A; Research Institute of Agriculture and Applied Science, 2356 Tokha, Kathmandu, Nepal.
  • Alharb SA; Department of Botany and Microbiology Department, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia.
  • Lakshmanaperumalsamy P; Department of Botany and Microbiology Department, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia.
Article en En | MEDLINE | ID: mdl-35368761
Oxytetracycline (OTC) which is a broad-spectrum veterinary tetracycline antibiotic is extensively used in poultry farms as a prophylactic, therapeutic, and growth stimulator. Upon administration, unmetabolized OTC is excreted from the animal body through droppings and accumulated in litter in the poultry industry. This study aimed at investigating the OTC degradation potential of an-OTC tolerant bacterial strain, isolated from poultry manure. The isolated strain's morphology, biochemical properties, and 16S ribosomal RNA (rRNA) gene sequence confirmed that it belonged to the Lysinibacillus genus. To measure the residual OTC concentration, a high-performance liquid chromatography method was used. OTC degradation rates were 2.579 mg L-1d-1 with Lysinibacillus strain 3+I and 1.149 mg L-1d-1 without Lysinibacillus strain 3+I. In the presence of strain 3+I, the half-life significantly reduced to 2.68 days, compared to 6.03 days without strain 3+I. The strain demonstrated 85% removal with the OTC concentration of 10 µg/ml. The influence of pH, temperature, carbon sources, and nitrogen source, which influence degradation, were also investigated. The optimum condition favouring degradation was pH 6 at a temperature of 30°C. In addition, Lysinibacillus sp. strain 3+I's ability to degrade OTC in poultry litter offers a promising approach to treat poultry manure and effluent containing OTC, preventing its contamination in the environment.

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Evid Based Complement Alternat Med Año: 2022 Tipo del documento: Article País de afiliación: India Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Evid Based Complement Alternat Med Año: 2022 Tipo del documento: Article País de afiliación: India Pais de publicación: Estados Unidos