Combination of engineering the substrate and Ca<sup>2+</sup> binding domains of heparinase I to improve the catalytic activity.

Zhou, Hua-Ping; Wang, Ding-Ran; Xu, Chen-Lu; Zhang, Ye-Wang

Combination of engineering the substrate and Ca²⁺ binding domains of heparinase I to improve the catalytic activity.

Zhou, Hua-Ping; Wang, Ding-Ran; Xu, Chen-Lu; Zhang, Ye-Wang.

Afiliación

Zhou HP; School of Pharmacy, Jiangsu University, Zhenjiang, P.R. China.
Wang DR; School of Pharmacy, Jiangsu University, Zhenjiang, P.R. China.
Xu CL; School of Pharmacy, Jiangsu University, Zhenjiang, P.R. China.
Zhang YW; School of Pharmacy, Jiangsu University, Zhenjiang, P.R. China.

Prep Biochem Biotechnol ; 53(10): 1297-1305, 2023 Nov.

Article en En | MEDLINE | ID: mdl-37040156

HighlightsImproved catalytic activity of heparinase I by engineering the binding domains of substrate and Ca²⁺.The mutant D152S/R244K/T250D showed the highest catalytic performance.The increased hydrogen bonds attribute to the increased activity.

Asunto(s)

Heparina de Bajo-Peso-Molecular; Heparina; Liasa de Heparina/química; Simulación del Acoplamiento Molecular; Heparina/química; Mutación

Palabras clave

Binding domain; catalytic activity; heparinase I; hydrogen bonds; molecular docking

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Heparina / Heparina de Bajo-Peso-Molecular Idioma: En Revista: Prep Biochem Biotechnol Asunto de la revista: BIOQUIMICA / BIOTECNOLOGIA Año: 2023 Tipo del documento: Article Pais de publicación: Reino Unido

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