Effects of Dietary Supplementation of arginine-silicate- Inositol and Phytase Complex on Egg Quality, Egg Shell Strength, and Blood Biochemical Characteristics of Laying Hens.

This study aimed to determine the effects of Arginine silicate inositol complex (ASI; Arg=49.47 %, silicone=8.2 %, inositol=25%) supplementation on egg quality, shell strength, and blood biochemical traits of laying hens, as well as the effects of substituting inositol with varying concentrations of phytase on the traits as mentioned above. 90 Lohmann Brown laying hens, 26 weeks old, were randomly distributed in 6 treatments with 3 replicates (cage) and 5 birds per replicate. The isocaloric and isonitrogenic diets are used according to the age period requirements of the Lohmann Brown Classic management guideline. The treatments were as follows: 1ST treatment T1: received basal diet without additives, T2 received basal diet +1000 mg/kg arginine-silicate mixture (49.5±8.2 % respectively), T3 received basal diet +1000 mg/kg arginine-silicate- inositol (ASI) mixture (49.5, 8.2 , 25 % respectively), T4 received basal diet +1000 mg/kg arginine-silicate mixture (49.5±8.2% respectively) +500 FTU/kg, T5 received basal diet +1000 mg/kg arginine-silicate mixture (49.5±8.2% respectively) +1000 FTU/kg and T6 received basal diet +1000 mg/kg arginine-silicate mixture (49.5±8.2% respectively) +1000 FTU/kg +2000 FTU/kg. Results indicate a significant increase (P<0.05) in the relative yolk weight in T4, T5, and T6 (26.93, 26.83, 26.77%) compared to T1 (25.84%) and a significant increase (P≤ 0.05) in T4, T5 compared to T3 (26.02%), while no differences observed between T2 (26.17%) compared to other experimental treatments. The relative albumin weight significantly decreased (P≤0.05) in phytase supplementation treatments T4, T5, and T6 (63.21, 63.05, 63.22%) compared to T1, T2, T3 (64.99, 64.30, 64.08%), while a significant decrease (P≤0.05) observed in T3 compared to T1. The relative shell weight significantly increased (P≤0.05) in T3, T4, T5, and T6 (9.90, 9.86, 10.12, 10.02%), respectively, compared to T1, T2 (9.17, 9.53%) with a significant increase (P≤0.05) in relative shell weight in T2 compared to T1. The eggshell thickness significantly increased (P≤0.05) in T3, T4, T5, T6 treatments (0.409, 0.408, 0.411, 0.413 mm), respectively compared to T1, T2 (0.384, 0.391 mm). A significant increased (P≤0.05) was observed in eggshell thickness in T2 compared to T1. A significant increase (P≤0.05) was observed in the egg shell breaking strength in T3 and T5 treatments (59.40, 58.83) compared to T1 and T2 (46.20, 48.23). No significant differences were observed between T4 and T6 (53.90, 53.57) compared to other experimental treatments. Non HDL, calcium, and phosphorus levels in blood serum significantly increased (P≤0.05) in T3, T4, T5, and T6 treatments compared to T1 and T2 treatments.