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Follicular Fluid-Derived Extracellular Vesicles Influence on In Vitro Maturation of Equine Oocyte: Impact on Cumulus Cell Viability, Expansion and Transcriptome.
Gabrys, Julia; Gurgul, Artur; Szmatola, Tomasz; Kij-Mitka, Barbara; Andronowska, Aneta; Karnas, Elzbieta; Kucharski, Miroslaw; Wojciechowska-Puchalka, Joanna; Kochan, Joanna; Bugno-Poniewierska, Monika.
Afiliación
  • Gabrys J; Department of Animal Reproduction, Anatomy and Genomics, Faculty of Animal Science, University of Agriculture in Krakow, Mickiewicza 24/28, 30-059 Krakow, Poland.
  • Gurgul A; Center for Experimental and Innovative Medicine, University of Agriculture in Krakow, Redzina 1c, 30-248 Krakow, Poland.
  • Szmatola T; Center for Experimental and Innovative Medicine, University of Agriculture in Krakow, Redzina 1c, 30-248 Krakow, Poland.
  • Kij-Mitka B; Department of Animal Reproduction, Anatomy and Genomics, Faculty of Animal Science, University of Agriculture in Krakow, Mickiewicza 24/28, 30-059 Krakow, Poland.
  • Andronowska A; Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Tuwima 10, 10-748 Olsztyn, Poland.
  • Karnas E; Department of Cell Biology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30-387 Krakow, Poland.
  • Kucharski M; Department of Animal Physiology and Endocrinology, University of Agriculture in Krakow, Mickiewicza 24/28, 30-059 Krakow, Poland.
  • Wojciechowska-Puchalka J; Department of Animal Reproduction, Anatomy and Genomics, Faculty of Animal Science, University of Agriculture in Krakow, Mickiewicza 24/28, 30-059 Krakow, Poland.
  • Kochan J; Department of Animal Reproduction, Anatomy and Genomics, Faculty of Animal Science, University of Agriculture in Krakow, Mickiewicza 24/28, 30-059 Krakow, Poland.
  • Bugno-Poniewierska M; Department of Animal Reproduction, Anatomy and Genomics, Faculty of Animal Science, University of Agriculture in Krakow, Mickiewicza 24/28, 30-059 Krakow, Poland.
Int J Mol Sci ; 25(6)2024 Mar 13.
Article en En | MEDLINE | ID: mdl-38542236
ABSTRACT
Cumulus cell (CC) expansion is pivotal for oocyte maturation, during which CCs release factors that initiate paracrine signaling within the follicular fluid (FF). The FF is abundant in extracellular vesicles (EVs) that facilitate intercellular communication. Although bovine and murine EVs can control cumulus expansion, these effects have not been observed in equines. This study aimed to assess the impact of FF-derived EVs (ffEVs) on equine CC expansion, viability, and transcriptome. Cumulus-oocyte complexes (COCs) that underwent in vitro maturation (IVM) in the presence (200 µg protein/mL) or absence (control) of ffEVs were assessed for cumulus expansion and viability. CCs were isolated after 12 h of IVM, followed by RNA extraction, cDNA library generation, and subsequent transcriptome analysis using next-generation sequencing. Confocal microscopy images illustrated the internalization of labeled ffEVs by CCs. Supplementation with ffEVs significantly enhanced cumulus expansion in both compacted (Cp, p < 0.0001) and expanded (Ex, p < 0.05) COCs, while viability increased in Cp groups (p < 0.01), but decreased in Ex groups (p < 0.05), compared to the controls. Although transcriptome analysis revealed a subtle effect on CC RNA profiles, differentially expressed genes encompassed processes (e.g., MAPK and Wnt signaling) potentially crucial for cumulus properties and, consequently, oocyte maturation.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Líquido Folicular / Vesículas Extracelulares Límite: Animals Idioma: En Revista: Int J Mol Sci Año: 2024 Tipo del documento: Article País de afiliación: Polonia Pais de publicación: Suiza

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Líquido Folicular / Vesículas Extracelulares Límite: Animals Idioma: En Revista: Int J Mol Sci Año: 2024 Tipo del documento: Article País de afiliación: Polonia Pais de publicación: Suiza