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Aptamer-aided plasmonic nano-urchins for reporter-free surface-enhanced Raman spectroscopy analysis of cortisol.
Li, Chengyu; Hu, Jing; Hu, Nan; Zhao, Jianjun; Li, Qianwen; Han, Yanhui; Liu, Yanxiong; Hu, Xufang; Zheng, Liyan; Cao, Qiue.
Afiliación
  • Li C; School of Chemical Science and Technology, National Demonstration Center for Experimental Chemistry and Chemical Engineering Education, Yunnan University, Kunming, Yunnan, 650091, China. huxufang@ynu.edu.cn.
  • Hu J; Clinical Psychology Department, Zhongshan Third People's Hospital, Zhongshan, Guangdong, 528451, China.
  • Hu N; School of Chemical Science and Technology, National Demonstration Center for Experimental Chemistry and Chemical Engineering Education, Yunnan University, Kunming, Yunnan, 650091, China. huxufang@ynu.edu.cn.
  • Zhao J; School of Chemical Science and Technology, National Demonstration Center for Experimental Chemistry and Chemical Engineering Education, Yunnan University, Kunming, Yunnan, 650091, China. huxufang@ynu.edu.cn.
  • Li Q; School of Chemical Science and Technology, National Demonstration Center for Experimental Chemistry and Chemical Engineering Education, Yunnan University, Kunming, Yunnan, 650091, China. huxufang@ynu.edu.cn.
  • Han Y; School of Chemical Science and Technology, National Demonstration Center for Experimental Chemistry and Chemical Engineering Education, Yunnan University, Kunming, Yunnan, 650091, China. huxufang@ynu.edu.cn.
  • Liu Y; School of Chemical Science and Technology, National Demonstration Center for Experimental Chemistry and Chemical Engineering Education, Yunnan University, Kunming, Yunnan, 650091, China. huxufang@ynu.edu.cn.
  • Hu X; School of Chemical Science and Technology, National Demonstration Center for Experimental Chemistry and Chemical Engineering Education, Yunnan University, Kunming, Yunnan, 650091, China. huxufang@ynu.edu.cn.
  • Zheng L; School of Chemical Science and Technology, National Demonstration Center for Experimental Chemistry and Chemical Engineering Education, Yunnan University, Kunming, Yunnan, 650091, China. huxufang@ynu.edu.cn.
  • Cao Q; School of Chemical Science and Technology, National Demonstration Center for Experimental Chemistry and Chemical Engineering Education, Yunnan University, Kunming, Yunnan, 650091, China. huxufang@ynu.edu.cn.
Anal Methods ; 16(19): 3067-3073, 2024 May 16.
Article en En | MEDLINE | ID: mdl-38683672
ABSTRACT
Cortisol is a vital glucocorticoid hormone reflecting stress levels and related disease processes. In this study, we report an aptamer-functionalized plasmonic nano-urchin (α-FeOOH@Au-aptamer)-aided cortisol-capturing and surface-enhanced Raman spectroscopy (SERS) analysis approach. The designed α-FeOOH@Au-aptamer exhibits a well-patterned plasma structure, which combines the good SERS enhancement ability of reduced nanogaps between the Au plasma and the hot spot-favored structure of anisotropic tips from α-FeOOH urchins, with the high affinity of the aptamer towards cortisol molecules. The α-FeOOH@Au-aptamer achieved reporter-free SERS quantification for cortisol with good sensitivity (limit of detection <0.28 µmol L-1), robust salt (1.0 mol per L NaCl) and protein (5.0 mg per mL bovine serum protein) tolerance, favorable reproducibility, as well as good reusability. We further demonstrated the good cortisol-capturing ability and SERS efficacy of the α-FeOOH@Au-aptamer profiling in the serum and urine samples. Our approach provides an alternative tool for cortisol analysis and a reference strategy for report-free SERS detection of small molecules.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Espectrometría Raman / Hidrocortisona / Aptámeros de Nucleótidos / Oro Límite: Animals / Humans Idioma: En Revista: Anal Methods Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Espectrometría Raman / Hidrocortisona / Aptámeros de Nucleótidos / Oro Límite: Animals / Humans Idioma: En Revista: Anal Methods Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido