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Establishment of genome-editing system and assembly of a near-complete genome in broomcorn millet.
Liu, Yang; Cheng, Zixiang; Chen, Weiyao; Wu, Chuanyin; Chen, Jinfeng; Sui, Yi.
Afiliación
  • Liu Y; State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, the Chinese Academy of Sciences, Beijing, 100101, China.
  • Cheng Z; State Key Laboratory of Crop Gene Resources and Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
  • Chen W; State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, the Chinese Academy of Sciences, Beijing, 100101, China.
  • Wu C; University of Chinese Academy of Sciences, Beijing, 100049, China.
  • Chen J; State Key Laboratory of Crop Gene Resources and Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
  • Sui Y; State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, the Chinese Academy of Sciences, Beijing, 100101, China.
J Integr Plant Biol ; 66(8): 1688-1702, 2024 Aug.
Article en En | MEDLINE | ID: mdl-38695644
ABSTRACT
The ancient crop broomcorn millet (Panicum miliaceum L.) is an indispensable orphan crop in semi-arid regions due to its short life cycle and excellent abiotic stress tolerance. These advantages make it an important alternative crop to increase food security and achieve the goal of zero hunger, particularly in light of the uncertainty of global climate change. However, functional genomic and biotechnological research in broomcorn millet has been hampered due to a lack of genetic tools such as transformation and genome-editing techniques. Here, we successfully performed genome editing of broomcorn millet. We identified an elite variety, Hongmi, that produces embryogenic callus and has high shoot regeneration ability in in vitro culture. We established an Agrobacterium tumefaciens-mediated genetic transformation protocol and a clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9-mediated genome-editing system for Hongmi. Using these techniques, we produced herbicide-resistant transgenic plants and edited phytoene desaturase (PmPDS), which is involved in chlorophyll biosynthesis. To facilitate the rapid adoption of Hongmi as a model line for broomcorn millet research, we assembled a near-complete genome sequence of Hongmi and comprehensively annotated its genome. Together, our results open the door to improving broomcorn millet using biotechnology.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Genoma de Planta / Sistemas CRISPR-Cas / Edición Génica / Panicum Idioma: En Revista: J Integr Plant Biol Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación:

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Genoma de Planta / Sistemas CRISPR-Cas / Edición Génica / Panicum Idioma: En Revista: J Integr Plant Biol Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: