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Generation of Rare Human NMDA Receptor Variants in Mice.
Sprengel, Rolf; Eltokhi, Ahmed; Single, Frank N.
Afiliación
  • Sprengel R; Max Planck Institute for Medical Research, Heidelberg, Germany. sprengel@mr.mpg.de.
  • Eltokhi A; Department of Biomedical Sciences, School of Medicine, Mercer University, Columbus, GA, USA.
  • Single FN; Miltenyi Biotec B.V. & Co. KG, Bergisch Gladbach, Germany.
Methods Mol Biol ; 2799: 79-105, 2024.
Article en En | MEDLINE | ID: mdl-38727904
ABSTRACT
The analysis of rare NMDAR gene variants in mice, coupled with a fundamental understanding of NMDAR function, plays a crucial role in achieving therapeutic success when addressing NMDAR dysfunctions in human patients. For the generation of such NMDAR mouse models, a basic knowledge of receptor structure, along with skills in database sequence analysis, cloning in E. coli, genetic manipulation of embryonic stem (ES) cells, and ultimately the genetic modification of mouse embryos, is essential. Primarily, this chapter will focus on the design and synthesis of NMDAR gene-targeting vectors that can be used successfully for the genetic manipulation of mice. We will outline the core principles of the design and synthesis of a gene targeting vector that facilitates the introduction of single-point mutations in NMDAR-encoding genes in mice. The transformation of ES cells, selection of positive ES cell colonies, manipulation of mouse embryos, and genotyping strategies will be described briefly.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Receptores de N-Metil-D-Aspartato Límite: Animals / Humans Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2024 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Receptores de N-Metil-D-Aspartato Límite: Animals / Humans Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2024 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Estados Unidos