Your browser doesn't support javascript.
loading
Development of a multiplex real-time PCR assay for the simultaneous detection of mpox virus and orthopoxvirus infections.
Fan, Zhangling; Xie, Yu; Huang, Baoying; Zhao, Fei; Hu, Yamei; Huang, Yu; Mei, Shan; Wei, Liang; Wang, Liming; Wang, Lingwa; Gao, Zhao; Ai, Bin; Fang, Jugao; Liang, Chen; Xu, Fengwen; Tan, Wenjie; Guo, Fei.
Afiliación
  • Fan Z; NHC Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, and Center for AIDS Research, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730, PR China.
  • Xie Y; NHC Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, and Center for AIDS Research, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730, PR China.
  • Huang B; NHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, PR China.
  • Zhao F; NHC Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, and Center for AIDS Research, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730, PR China.
  • Hu Y; NHC Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, and Center for AIDS Research, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730, PR China.
  • Huang Y; NHC Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, and Center for AIDS Research, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730, PR China.
  • Mei S; NHC Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, and Center for AIDS Research, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730, PR China.
  • Wei L; NHC Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, and Center for AIDS Research, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730, PR China.
  • Wang L; NHC Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, and Center for AIDS Research, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730, PR China.
  • Wang L; Department of Otolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, PR China.
  • Gao Z; NHC Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, and Center for AIDS Research, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730, PR China.
  • Ai B; Department of Medical Oncology, Beijing Hospital, National Center of Gerontology, Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, Beijing 100730, PR China.
  • Fang J; Department of Otolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, PR China.
  • Liang C; Lady Davis Institute, Jewish General Hospital, McGill University, Montreal, Quebec H3T 1E2, Canada.
  • Xu F; NHC Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, and Center for AIDS Research, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730, PR China. Electronic address: xanadufw@aliyun.com.
  • Tan W; NHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, PR China. Electronic address: tanwj@ivdc.chinacdc.cn.
  • Guo F; NHC Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, and Center for AIDS Research, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730, PR China. Electronic address: guofei@ipb.pumc.edu.cn.
J Virol Methods ; 328: 114957, 2024 Jul.
Article en En | MEDLINE | ID: mdl-38788978
ABSTRACT
Since May 2022, the multi-country outbreak of monkeypox (mpox) has raised a great concern worldwide. Early detection of mpox virus infection is recognized as an efficient way to prevent mpox transmission. Mpox specific detection methods reported up to now are based on the SNPs among mpox virus and other orthopoxviruses. We have therefore developed a real-time PCR based mpox detection method targeting mpox virus specific sequences (N3R and B18Rplus). We have also optimized an orthopoxvirus detection system which targets the highly conserved E9L and D6R genes. The mpox and orthopoxvirus real-time PCR assays have a high sensitivity (1 copy/reaction) and specificity. Mpox viral DNA and clinical samples from mpox patients are detected with the mpox detection system. Furthermore, we have established a multiplex real-time PCR detection system allowing simultaneous and efficient detection of mpox and orthopoxvirus infections.
Asunto(s)
Palabras clave

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Sensibilidad y Especificidad / Orthopoxvirus / Monkeypox virus / Infecciones por Poxviridae / Mpox / Reacción en Cadena de la Polimerasa Multiplex / Reacción en Cadena en Tiempo Real de la Polimerasa Límite: Humans Idioma: En Revista: J Virol Methods Año: 2024 Tipo del documento: Article Pais de publicación: Países Bajos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Sensibilidad y Especificidad / Orthopoxvirus / Monkeypox virus / Infecciones por Poxviridae / Mpox / Reacción en Cadena de la Polimerasa Multiplex / Reacción en Cadena en Tiempo Real de la Polimerasa Límite: Humans Idioma: En Revista: J Virol Methods Año: 2024 Tipo del documento: Article Pais de publicación: Países Bajos