Islet cell antigen 512 is a diabetes-specific islet autoantigen related to protein tyrosine phosphatases.
J Immunol
; 152(6): 3183-8, 1994 Mar 15.
Article
en En
| MEDLINE
| ID: mdl-8144912
ABSTRACT
Islet cell Ag 512 (ICA512) is a recombinant human Ag that was isolated from an islet cDNA expression library by screening with human insulin-dependent diabetes mellitus sera. Specificity of reaction with diabetic sera was demonstrated initially by immunoprecipitation with a small number of diabetic and normal serum samples. To permit quantitative and rapid serum testing, ICA512 was purified and adapted to an ELISA format. In this way, a sensitivity of 48% with newly diagnosed diabetic sera has been measured with a panel of 80 sera. DNA sequencing of ICA512-3, a cDNA that contains a 1644 bp open reading frame, suggests that it codes for a transmembrane protein having a single membrane-spanning segment and a cytoplasmic domain that is closely related to the first intracellular (catalytic) domain of the T cell protein tyrosine phosphatase, CD45. Northern blot analysis of poly(A)+ RNAs from several human tissues indicates that ICA512 mRNA is expressed in brain and pancreas.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Autoantígenos
/
Islotes Pancreáticos
/
Proteínas Tirosina Fosfatasas
/
Diabetes Mellitus
Límite:
Humans
Idioma:
En
Revista:
J Immunol
Año:
1994
Tipo del documento:
Article