Nucleotide sequence and characterization of the Rhodobacter capsulatus hvrB gene: HvrB is an activator of S-adenosyl-L-homocysteine hydrolase expression and is a member of the LysR family.
J Bacteriol
; 176(1): 61-9, 1994 Jan.
Article
en En
| MEDLINE
| ID: mdl-8282711
ABSTRACT
Here we present the nucleotide sequence and characterization of two genes, hvrB and orf5, that are located in the regulatory gene cluster from Rhodobacter capsulatus. The hvrB gene, which encodes a protein with a predicted molecular mass of 32 kDa, is shown to be highly homologous to genes encoding members of the LysR family of bacterial transcriptional regulators. A chromosomal disruption of hvrB is shown to result in the failure to regulate expression from the nearby ahcY and orf5 genes in response to alterations in light intensity. We show by primer extension mapping that the 5' end of ahcY-specific mRNA defines a promoter region exhibiting sequence similarity to known R. capsulatus promoter elements. Our mutational analysis further demonstrates that hvrB autoregulates its own expression in vivo.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteínas Bacterianas
/
Regulación Bacteriana de la Expresión Génica
/
Genes Reguladores
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Rhodobacter capsulatus
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Activación Enzimática
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Genes Bacterianos
/
Hidrolasas
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
J Bacteriol
Año:
1994
Tipo del documento:
Article