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Immunochemical study of a Paracoccidioides brasiliensis polysaccharide-like antigen.
Mendes-Giannini, M J; Toscano, E; del Negro, G B; Assis, C M; Garcia, N M.
Afiliación
  • Mendes-Giannini MJ; Department of Clinical Analysis, Faculty of Pharmaceutical Sciences-UNESP, Araraquara, São Paulo, Brazil.
J Med Vet Mycol ; 33(6): 379-83, 1995.
Article en En | MEDLINE | ID: mdl-8683406
The polysaccharide antigen from P. brasiliensis has been largely employed in serologic tests ,as well as in skin tests, to evaluate cellular immunity. SDS-PAGE analysis of this antigen has revealed a variability in the number of bands exhibited by isolates SN, 265, 339, 113, and 18 (7 to 16 bands). The antigens obtained from isolates 2, PTL, 192 and Adel showed two or three bands. Glycoprotein analysis demonstrated a broad region between 50 and 90 kDa. Major bands of 48 and 30 kDa were present in almost all antigens. Optimal complement fixing dilution appears to be unaffected by the number of bands presented by different antigens. The immunoblot analysis revealed that the 90 and 30 kDa bands were mainly recognized by sera from paracoccidioidomycosis patients. Bands of high molecular weight were also recognized by most of the sera studied. Sera from histoplasmosis recognized the 94 kDa band. In conclusion, although the isolates exhibit quantitative variability in the number of fractions, it is possible to use only one or two samples given the greatest frequency of reactivity is seen in the 30 and 90 kDa fractions.
Asunto(s)
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Paracoccidioides / Paracoccidioidomicosis / Antígenos Fúngicos Límite: Humans País/Región como asunto: America do sul / Brasil Idioma: En Revista: J Med Vet Mycol Asunto de la revista: MICROBIOLOGIA Año: 1995 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Reino Unido
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Paracoccidioides / Paracoccidioidomicosis / Antígenos Fúngicos Límite: Humans País/Región como asunto: America do sul / Brasil Idioma: En Revista: J Med Vet Mycol Asunto de la revista: MICROBIOLOGIA Año: 1995 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Reino Unido