Molecular detection of streptomycin-producing streptomycetes in Brazilian soils.
Appl Environ Microbiol
; 63(4): 1288-97, 1997 Apr.
Article
en En
| MEDLINE
| ID: mdl-9097426
ABSTRACT
Actinomycetes were isolated from soybean rhizosphere soil collected as two field sites in Brazil. All the isolates were identified as Streptomyces species and were screened for streptomycin production and the presence of two genes, strA and strB1, known to be involved in streptomycin biosynthesis in Streptomyces griseus. Antibiotic resistance profiles were determined for 53 isolates from cultivated and uncultivated sites, and approximately half the strains were streptomycin resistance. Clustering by the unweighted pair group method with averages indicated the presence of two major clusters, with the majority of resistant strains from cultivated sites being placed in cluster 1. Only representatives from this cluster contained strA. Streptomycetes containing strA and strB1 were phenotypically diverse, and only half could be assigned to known species. Sequence comparison of 16S rRNA and trpBA (tryptophan synthetase) genes revealed that streptomycin- producing streptomycetes were phylogenetically diverse. It appeared that a population of streptomycetes had colonized the rhizosphere and that a proportion of these were capable of streptomycin production.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Microbiología del Suelo
/
Streptomyces
/
Estreptomicina
Tipo de estudio:
Diagnostic_studies
País/Región como asunto:
America do sul
/
Brasil
Idioma:
En
Revista:
Appl Environ Microbiol
Año:
1997
Tipo del documento:
Article
País de afiliación:
Reino Unido