Analysis of the puc operon promoter from Rhodobacter capsulatus.
J Bacteriol
; 180(16): 4270-7, 1998 Aug.
Article
en En
| MEDLINE
| ID: mdl-9696778
ABSTRACT
Expression of the Rhodobacter capsulatus puc operon, which codes for structural polypeptides of the light-harvesting-II peripheral antenna complex, is highly regulated in response to alterations in oxygen tension and light intensity. To obtain an understanding of the puc promoter region we report the high-resolution 5' mapping of the puc mRNA transcriptional start site and DNA sequence analysis of the puc upstream regulatory sequence (pucURS). A sigma70-type promoter sequence was identified (pucP1) which has a high degree of sequence similarity with carotenoid and bacteriochlorophyll biosynthesis promoters. Inspection of the DNA sequence also indicated the presence of two CrtJ and four integration host factor (IHF) binding sites. Transcriptional fusions of the pucURS fused to lacZ also confirmed that puc promoter activity is regulated by the transcriptional regulators IHF, CrtJ, and RegA. Gel retardation analysis using cell extracts indicates that mutations in IHF and RegA disrupt protein binding to DNA fragments containing the pucURS.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Operón
/
Regulación Bacteriana de la Expresión Génica
/
Rhodobacter capsulatus
/
Regiones Promotoras Genéticas
/
Proteínas del Complejo del Centro de Reacción Fotosintética
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
J Bacteriol
Año:
1998
Tipo del documento:
Article
País de afiliación:
Estados Unidos