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Reduced amplification efficiency of the RNA-dependent-RNA-polymerase (RdRp) target enables tracking of the Delta SARS-CoV-2 variant using routine diagnostic tests
Ziyaad Valley-Omar; Kruger Marais; Arash Iranzadeh; Michelle Naidoo; Stephen Korsman; Tongai Maponga; Hannah Hussey; Mary-Ann Davies; Andrew Boulle; Deelan Doolabh; Mariska Laubscher; JD Deetlefs; Jean Maritz; Lesley Scott; Nokukhanya Msomi; Houriiyah Tegally; Tulio de Oliveira; Jinal Bhiman; Carolyn Williamson; Wolfgang Preiser; Diana Ruth Hardie; Nei-yuan Hsiao.
Afiliación
  • Ziyaad Valley-Omar; Division of Medical Virology, University of Cape Town, Cape Town, Western Cape, South Africa
  • Kruger Marais; Division of Medical Virology, University of Cape Town, Cape Town, Western Cape, South Africa
  • Arash Iranzadeh; Division of Medical Virology, University of Cape Town, Cape Town, Western Cape, South Africa
  • Michelle Naidoo; Division of Medical Virology, University of Cape Town, Cape Town, Western Cape, South Africa
  • Stephen Korsman; Division of Medical Virology, University of Cape Town, Cape Town, Western Cape, South Africa
  • Tongai Maponga; Division of Medical Virology, Stellenbosch University, Western Cape, South Africa
  • Hannah Hussey; Centre Health Intelligence, Western Cape Government: Health, South Africa
  • Mary-Ann Davies; Centre for Infectious Disease Epidemiology and Research, School of Public health, University of Cape Town, Western Cape South Africa
  • Andrew Boulle; Centre for Infectious Disease Epidemiology and Research, School of Public health, University of Cape Town, Western Cape South Africa
  • Deelan Doolabh; Division of Medical Virology, University of Cape Town, Cape Town, Western Cape, South Africa
  • Mariska Laubscher; Lancet laboratories, South Africa
  • JD Deetlefs; Ampath Laboratories, South Africa
  • Jean Maritz; PathCare Laboratories, South Africa
  • Lesley Scott; Research diagnostic Laboratories, School of Pathology, University of the Witwatersrand, South Africa
  • Nokukhanya Msomi; Discipline of Virology, University of KwaZulu-Natal, Durban, South Africa
  • Houriiyah Tegally; KwaZulu-Natal Research, Innovation & Sequencing Platform, University of KwaZulu-Natal, Durban, South Africa
  • Tulio de Oliveira; KwaZulu-Natal Research, Innovation & Sequencing Platform, University of KwaZulu-Natal, Durban, South Africa
  • Jinal Bhiman; National institute for Communicable Diseases, Johannesburg, South Africa
  • Carolyn Williamson; Division of Medical Virology, University of Cape Town, Cape Town, Western Cape, South Africa
  • Wolfgang Preiser; Division of Medical Virology, Stellenbosch University, Western Cape, South Africa
  • Diana Ruth Hardie; Division of Medical Virology, University of Cape Town, Cape Town, Western Cape, South Africa
  • Nei-yuan Hsiao; Division of Medical Virology, University of Cape Town, Cape Town, Western Cape, South Africa
Preprint en Inglés | medRxiv | ID: ppmedrxiv-21264408
ABSTRACT
Routine SARS-CoV-2 surveillance in the Western Cape region of South Africa (January-August 2021) found a reduced PCR amplification efficiency of the RdRp gene target of the Seegene, Allplex 2019-nCoV diagnostic assay when detecting the Delta variant. We propose that this can be used as a surrogate for variant detection.
Licencia
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Texto completo: Disponible Colección: Preprints Base de datos: medRxiv Tipo de estudio: Estudio diagnóstico Idioma: Inglés Año: 2021 Tipo del documento: Preprint
Texto completo: Disponible Colección: Preprints Base de datos: medRxiv Tipo de estudio: Estudio diagnóstico Idioma: Inglés Año: 2021 Tipo del documento: Preprint
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