Azul de toluidina como método para avaliação da integridade da cromatina de espermatozoides canino extraídos do tecido testicular fresco / Toluidine blue as a method for assessment of chromatin integrity canine spermatozoa extracted from fresh testicular tissue

ABSTRACT

Conservation of gonadal tissue is important for maintenance of endangered wild species. However, thepreservation can lead to cell damage and loss. Thus, it is necessary testicular tissue evaluation before and afterstorage in order to determine damage. The evaluation of sperm chromatin integrity ensures a better assessmentof sperm quality. The toluidine blue is a simple and sensitive test to evaluate the sperm chromatin integrity fromseveral species, but there are not reports in the dog. The aim of this study was evaluating canine spermatozoaextracted from fresh testicular tissue of seven post-pubertal mongrel dogs by toluidine blue and spermmorphology. For statistical analysis, it was used normality test (Shapiro Wilk) and Wilcoxon test (P<0.05).Toluidine blue showed 83% condensed chromatin. Morphological analysis presented 69.34% normalspermatozoa. Toluidine blue was sensitive for the evaluation. However, it still needs some adjustments for amore accurate result for such specie.