Multi-component comprehensive quality evaluation of Tongjing ointment / 中国药师
China Pharmacist
; (12): 319-331, 2023.
Article
en Zh
| WPRIM
| ID: wpr-1025886
Biblioteca responsable:
WPRO
ABSTRACT
Objective To establish a method for comprehensive quality evaluation of Tongjing ointment by multi-component quantification combined with chemometrics and grey correlation analysis(GRA).Methods With curcumin as the internal reference substance,HPLC-QAMS method was used to simultaneously determine the contents of limonin,evodiamine,rutaecarpine,bisdemethoxycurcumin,demethoxycurcumin,curcumin,6-gingerol,8-gingerol,10-gingerol,tetrahydropalmatine,dehydrocorydalin,dorydaline in Tongjing ointment.The quality of Tongjing ointment was evaluated by cluster analysis,principal component analysis,orthogonal partial least square discriminant analysis and GRA.Results The determination of 12 components manifested a good linear relationship in the range of mass concentration(r≥0.999 1).The average recovery was between 96.58%and 100.19%(RSD<2.0%,n=9).There were no significant difference between the measured value of external standard method and the calculated value of HPLC-QAMS(P>0.05).Tongjing ointment samples were classified into three eategories by chemometrics and it showed that curcumin,6-gingerol,bisdemethoxycurcumin,demethoxycurcumin,limonin and tetrahydropalmatine were the main potential markers affecting the quality of Tongjing ointment.GRA showed that the relative correlation degree was in the range of 0.317 3-0.624 8,and there were some differences in the quality of Tongjing ointment.Conclusion The established method can comprehensively evaluate the quality of Tongjing ointment.
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Base de datos:
WPRIM
Idioma:
Zh
Revista:
China Pharmacist
Año:
2023
Tipo del documento:
Article