Identification of a Calcium-activated Potassium Channel Gene Expressed in Rat Cardiac Myocytes
Journal of the Korean Pediatric Society
; : 905-912, 2000.
Article
en Ko
| WPRIM
| ID: wpr-113893
Biblioteca responsable:
WPRO
ABSTRACT
PURPOSE: Calcium-activated potassium channels(KCa) may be involved in the transient outward current of the first phase of cardiac action potential. But it is still not clear whether cardiac myocytes express any Kca. We try to identify here the types of Kc, expressed in rat caridac myocytes. METHODS: We isolated total heart RNA from 50 rats(Spague-Dawley) and performed reverse transcription-polymerase chain reaction(RT-PCR) using specifically designed synthetic oligonucleotide primer sets. From the pure culture of cardiac myocyte, Kc, gene expression was detected by Southern blot analysis. RESULTS: RT-PCR revealed expressions of BKca(large-conductance Kca, rSlo) and S&,(small-conductance Kca, rSK1). We prepared cardiac myocytes pure culture(>9596 pure myocyte) using pure culture technique. RT-PCR and Southern blot analysis of rat cardiac myocyte showed only rSK1-specific band, but no rSlo-specific was detected. CONCLUSION: The expressions of more than one type of Kca are detected from rat heart. A sub-type of SKcrSK1, was expressed in cardiac myocyte, while the main subunit of BKca(rSlo) was found in cells other than myocytes, most likely in the smooth muscle of cardiac blood vessels.
Palabras clave
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Potasio
/
Vasos Sanguíneos
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Potenciales de Acción
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ARN
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Expresión Génica
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Southern Blotting
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Canales de Potasio Calcio-Activados
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Células Musculares
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Miocitos Cardíacos
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Técnicas de Cultivo
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
Idioma:
Ko
Revista:
Journal of the Korean Pediatric Society
Año:
2000
Tipo del documento:
Article