Anti-inflammatory Activity of Carpinus tschonoskii Leaves Extract in R848-stimulated Bone Marrow-derived Macrophages and Dendritic Cells
Journal of Bacteriology and Virology
; : 77-82, 2012.
Artículo
en Inglés
| WPRIM (Pacífico Occidental)
| ID: wpr-165302
Biblioteca responsable:
WPRO
ABSTRACT
The present study aims to evaluate the anti-inflammatory effect of methanol extract from leaves of Carpinus tschonoskii (CE) on R848-stimulated primary bone marrow-derived macrophages (BMDMs) and dendritic cells (BMDCs). Primary BMDMs and BMDCs were used for pro-inflammatory cytokine production. Human embryonic kidney cell line 293T (HEK293T) was used to access NF-kappaB activity. In all cases, R848 was used to stimulate the cells. The CE (0~150 microg/ml) was treated to BMDMs, BMDCs, and HEK293T cells. CE pre-treatment in R848-stimulated BMDMs and BMDCs showed a dose-dependent inhibitory effect on pro-inflammatory cytokine (e.g., IL-12 p40, IL-6, and TNF-alpha) production as compared to non-treated controls. In NF-kappaB reporter gene assay, the CE pre-treatment inhibited NF-kappaB-dependent luciferase activity in a dose-dependent manner. Overall, our findings suggest that CE has significant inhibitory effect on pro-inflammatory cytokine production and deserve further studies concerning potentials of CE for medicinal uses.
Texto completo:
Disponible
Base de datos:
WPRIM (Pacífico Occidental)
Asunto principal:
Células Dendríticas
/
Línea Celular
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FN-kappa B
/
Interleucina-6
/
Genes Reporteros
/
Interleucina-12
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Corynebacterium
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Betulaceae
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Metanol
/
Riñón
Límite:
Humanos
Idioma:
Inglés
Revista:
Journal of Bacteriology and Virology
Año:
2012
Tipo del documento:
Artículo