Brain-Derived Neurotrophic Factor (BDNF) Exerts a Protective Effect via an Anti-Apoptotic Mechanism on Hypoxic-Ischemic Injury in the Rat Brain / 대한주산의학회잡지
Korean Journal of Perinatology
; : 15-27, 2016.
Artículo
en Inglés
| WPRIM (Pacífico Occidental)
| ID: wpr-17700
Biblioteca responsable:
WPRO
ABSTRACT
PURPOSE:
Perinatal hypoxic-ischemic (HI) brain injury remains a common cause of chronic handicapping conditions of cerebral palsy, mental retardation, learning disability, and epilepsy. HI brain injury induces cell death via either necrosis or apoptosis. Brain-derived neurotrophic factor (BDNF) is a member of the neurotrophin family. It plays key roles in survival, differentiation, and maintenance of neurons. This study was to investigate the neuroprotective effects of BDNF via the mechanisms of anti-apoptosis in HI brain injury by using cortical astrocyte and neuronal cell culture.METHODS:
Cortical astrocytes culture of 1-day-old Sprague-Dawley (SD) rat pups and embryonic cortical neuronal cell culture of SD rats at 14-day gestation were done. The Normoxia group was prepared in 5% CO2 incubators and the Hypoxia group and Hypoxia+BDNF group (after treatment with BDNF for 24 hours) were placed in 1% O2 incubators (94% N2, 5% CO2) for 6 or 18 hours. The expression of Bcl-2 and Bax were assessed by real-time PCR and western blot. The caspase-3 activation was evaluated by caspase activity assay kit.RESULTS:
In astrocyte and neuronal cell, the expressions of Bcl-2 in the hypoxia groups were reduced compared to the normoxia groups, whereas, those in the Hypoxia+BDNF groups were increased compared to the hypoxia groups. However, the expressions of Bax and caspase-3 and the ratio of Bax/Bcl-2 were revealed reversely. In astrocyte, Hypoxia group for 6 hours was not significantly altered in Bcl-2, Bax expressions.CONCLUSION:
BDNF neuroprotective effects on HI brain injury in neonatal rats may occur via anti-apoptotic mechanism.
Texto completo:
Disponible
Contexto en salud:
ODS3 - Salud y Bienestar
/
ODS3 - Meta 3.4 Reducir las muertes prematuras por enfermedades no transmisibles
/
ODS3 - Meta 3.2 Evitar muertes en recién nacidos y niños menores de 5 años
Problema de salud:
Meta 3.2: Evitar muertes en recién nacidos y niños menores de 5 años
/
Epilepsia
/
Salud Mental y Trastornos del Comportamiento
/
Atención de Salud Neonatal
/
Enfermedades No Transmisibles
Base de datos:
WPRIM (Pacífico Occidental)
Asunto principal:
Encéfalo
/
Lesiones Encefálicas
/
Parálisis Cerebral
/
Western Blotting
/
Astrocitos
/
Muerte Celular
/
Ratas Sprague-Dawley
/
Apoptosis
/
Fármacos Neuroprotectores
/
Técnicas de Cultivo de Célula
Límite:
Animales
/
Humanos
/
Embarazo
Idioma:
Inglés
Revista:
Korean Journal of Perinatology
Año:
2016
Tipo del documento:
Artículo