Surface characteristics and osteoblastic cell response of alkali-and heat-treated titanium-8tantalum-3niobium alloy
Journal of Periodontal & Implant Science
; : 248-255, 2012.
Artículo
en Inglés
| WPRIM (Pacífico Occidental)
| ID: wpr-188637
Biblioteca responsable:
WPRO
ABSTRACT
PURPOSE:
The aim of the present study was to evaluate the biological response of alkali- and heat-treated titanium-8tantalum-3niobium surfaces by cell proliferation and alkaline phosphatase (ALP) activity analysis.METHODS:
Commercial pure titanium (group cp-Ti) and alkali- and heat-treated titanium-8tantalum-3niobium (group AHT) disks were prepared. The surface properties were evaluated using scanning electron microscopy, energy dispersed spectroscopy and X-ray photoelectron spectroscopy (XPS). The surface roughness was evaluated by atomic force microscopy and a profilometer. The contact angle and surface energy were also analyzed. The biological response of fetal rat calvarial cells on group AHT was assessed by cell proliferation and ALP activity.RESULTS:
Group AHT showed a flake-like morphology microprofile and dense structure. XPS analysis of group AHT showed an increased amount of oxygen in the basic hydroxyl residue of titanium hydroxide groups compared with group cp-Ti. The surface roughness (Ra) measured by a profilometer showed no significant difference (P>0.05). Group AHT showed a lower contact angle and higher surface energy than group cp-Ti. Cell proliferation on group AHT surfaces was significantly higher than on group cp-Ti surfaces (P<0.05). In comparison to group cp-Ti, group AHT enhanced ALP activity (P<0.05).CONCLUSIONS:
These results suggest that group AHT stimulates osteoblast differentiation.
Texto completo:
Disponible
Base de datos:
WPRIM (Pacífico Occidental)
Asunto principal:
Osteoblastos
/
Oxígeno
/
Análisis Espectral
/
Propiedades de Superficie
/
Titanio
/
Microscopía Electrónica de Rastreo
/
Adhesión Celular
/
Microscopía de Fuerza Atómica
/
Proliferación Celular
/
Fosfatasa Alcalina
Límite:
Animales
Idioma:
Inglés
Revista:
Journal of Periodontal & Implant Science
Año:
2012
Tipo del documento:
Artículo