Inhibitory effects of osteoprotegerin on osteoclast formation and function under serum-free conditions
Journal of Veterinary Science
; : 405-412, 2013.
Artículo
en Inglés
| WPRIM (Pacífico Occidental)
| ID: wpr-197113
Biblioteca responsable:
WPRO
ABSTRACT
The purpose of this study was to determine whether osteoprotegerin (OPG) could affect osteoclat differentiation and activation under serum-free conditions. Both duck embryo bone marrow cells and RAW264.7 cells were incubated with macrophage colony stimulatory factor (M-CSF) and receptor activator for nuclear factor kappaB ligand (RANKL) in serum-free medium to promote osteoclastogenesis. During cultivation, 0, 10, 20, 50, and 100 ng/mL OPG were added to various groups of cells. Osteoclast differentiation and activation were monitored via tartrate-resistant acid phosphatase (TRAP) staining, filamentous-actin rings analysis, and a bone resorption assay. Furthermore, the expression osteoclast-related genes, such as TRAP and receptor activator for nuclear factor kappaB (RANK), that was influenced by OPG in RAW264.7 cells was examined using real-time polymerase chain reaction. In summary, findings from the present study suggested that M-CSF with RANKL can promote osteoclast differentiation and activation, and enhance the expression of TRAP and RANK mRNA in osteoclasts. In contrast, OPG inhibited these activities under serum-free conditions.
Texto completo:
Disponible
Base de datos:
WPRIM (Pacífico Occidental)
Asunto principal:
Osteoclastos
/
Fosfatasa Ácida
/
Células de la Médula Ósea
/
Células Cultivadas
/
Factor Estimulante de Colonias de Macrófagos
/
Proteínas Aviares
/
Patos
/
Embrión no Mamífero
/
Ligando RANK
/
Receptor Activador del Factor Nuclear kappa-B
Límite:
Animales
Idioma:
Inglés
Revista:
Journal of Veterinary Science
Año:
2013
Tipo del documento:
Artículo