Kinetic Analysis of Na-K-ATPase Activity of Rabbit Cerebral Cortex by DDVP
Journal of Korean Neurosurgical Society
; : 17-22, 1982.
Artículo
en Coreano
| WPRIM (Pacífico Occidental)
| ID: wpr-21614
Biblioteca responsable:
WPRO
ABSTRACT
The kinetics of inhibition of Na-K-ATPase activity by o, 0-dimethyl o-(2,2-dichlorovinyl) phosphate(DDVP) was investigated with homogenerate of rabbit cerebral cortex. The results were summarized as the follows 1. Inhibition of Na-K-ATPase activity by DDVP showed a dose-dependent manner with an estimated I5.0 of 3.5x10(-4) M. 2. Altered pH and activity curves for Na-K-ATPase activity demonstrated comparable inhibition by DDVP in buffered acidic, neutral and alkaline pH renges. 3. Kinetic studies of cationic-substrate activation of Na-K-ATPase showed noncompetitive with respect to substrate and K+ and a mixed type inhibition with respect to Na+. These results suggest that DDVP appeared to exert its effects on Na-K-ATPase activity by interfering with the formation of Na-dependent phosphoenzyme.
Texto completo:
Disponible
Base de datos:
WPRIM (Pacífico Occidental)
Asunto principal:
Cinética
/
Corteza Cerebral
/
Diclorvos
/
Concentración de Iones de Hidrógeno
Idioma:
Coreano
Revista:
Journal of Korean Neurosurgical Society
Año:
1982
Tipo del documento:
Artículo