A single fraction from Uncaria sinensis exerts neuroprotective effects against glutamate-induced neurotoxicity in primary cultured cortical neurons / 대한해부학회지
Anatomy & Cell Biology
; : 95-103, 2015.
Article
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| WPRIM
| ID: wpr-23347
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WPRO
ABSTRACT
We identified a neuroprotective single fraction among 62 ones of hexane extract from Uncaria sinensis (JGH43IA) and investigated its effects and mechanisms in primary cortical neurons. Pretreatment with JGH43IA showed a significantly increase cell viability in a dose-dependent manner with a decrease in the lactate dehydrogenase release. When we performed morphological assay and flow cytometry to determination of the type of cell death, pretreatment with JGH43IA showed a significant reduction of glutamate-induced apoptotic cell death. Then we explored the downstream signaling pathways of N-methyl-D-aspartate receptor (NMDAR) with calpain activation to elucidate possible pathways of neuroprotection by JGH43IA. Pretreatment with JGH43IA exhibited a significant attenuation of NMDAR GluN2B subunit activation and a decrease in active form of calpain 1 leading to subsequent cleavage of striatal-enriched protein tyrosine phosphatase (STEP). In addition, pretreatment with JGH43IA showed a marked increase of cAMP responsive element binding protein. These results suggest that JGH43IA may have neuroprotective effects through down-regulation of NMDAR GluN2B subunit and calpain 1 activation, and subsequent alleviation of STEP cleavage. This single fraction from U. sinensis might be a useful therapeutic agent for brain disorder associated with glutamate injury.
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Base de datos:
WPRIM
Asunto principal:
Encefalopatías
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Calpaína
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Proteínas Portadoras
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Regulación hacia Abajo
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Supervivencia Celular
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N-Metilaspartato
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Proteínas Tirosina Fosfatasas
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Muerte Celular
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Receptores de N-Metil-D-Aspartato
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Fármacos Neuroprotectores
Idioma:
En
Revista:
Anatomy & Cell Biology
Año:
2015
Tipo del documento:
Article