Effect of YM155 on Apoptosis and Autophagy of K562 Cells / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 375-380, 2015.
Article
en Zh
| WPRIM
| ID: wpr-259582
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>This study was purposed to investigate the effect of YM155, a survivin inhibitor, on the apoptosis and autophagy of K562 cells.</p><p><b>METHODS</b>K562 cells were treated with YM155 at different concentration. Cell survival was analyzed by CCK-8 assay, the cell apoptosis was detected by flow cytometry. Survivin, BCL-2 and beclin1 mRNA expressions were determined by RT-PCR. Survivin, BCL-2, caspase-3, PARP and LC-3 protein expressions were assayed by Western blot.</p><p><b>RESULTS</b>YM155 inhibited the proliferation of K562 cells in a time- and dose-dependent manners. With the increasing of YM155 concentration and prolonging of action time, the expression levels of mRNA and protein of survivin and BCL-2 decreased, while the expression levels of caspase-3, PARP, beclin1 and LC-3 increased. Compared with the YM155 group, the protein levels of LC-3 and caspase-3 were lower in YM155 combined with 3-MA group.</p><p><b>CONCLUSION</b>YM155 can inhibit K562 cell proliferation by inducing apoptosis and autophagy, while autophagy induction effect can enhance its cytotoxic effect.</p>
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Autofagia
/
Naftoquinonas
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Apoptosis
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Células K562
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Proliferación Celular
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Citometría de Flujo
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Imidazoles
Límite:
Humans
Idioma:
Zh
Revista:
Journal of Experimental Hematology
Año:
2015
Tipo del documento:
Article