The role of peroxisome proliferators-activated receptor gamma (PPARgamma) during activation of hepatic stellate cells / 中华肝脏病杂志
Chinese Journal of Hepatology
; (12): 216-218, 2004.
Artículo
en Chino
| WPRIM (Pacífico Occidental)
| ID: wpr-260059
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVES</b>To observe the role of PPARgamma during the activation process of hepatic stellate cells (HSC).</p><p><b>METHODS</b>By morphology and RT-PCR, we study the changes of expression of PPARgamma in culture-activated HSC or in vivo activated HSC induced by dimethylnitrosamine (DMN).</p><p><b>RESULTS</b>In vitro, the expression level of PPARgamma in freshly isolated HSC (0.72+/-0.01) significantly reduced to 0.48+/-0.03 on the third day of culture (t = 19.8372, P<0.01), and reduced 70% on the seventh culture-day and could not be detected after the second passage. In vivo, HSC freshly isolated from normal control rats expressed PPARgamma (0.76+/-0.01). During the development of rat liver fibrosis induced by DMN, the expression level significantly reduced to 0.46+/-0.02 after the third injection of DMN (t = 29.5318, P<0.01), and reduced 66% on the end of first week and could not be detected on the end of second and third week.</p><p><b>CONCLUSION</b>The expression of PPARgamma might play an important role on the maintenance of resting-form of HSC, and the reduction of expression of PPARgamma might be an early event during the activation process of HSC.</p>
Texto completo:
Disponible
Base de datos:
WPRIM (Pacífico Occidental)
Asunto principal:
Patología
/
Fisiología
/
Factores de Transcripción
/
ARN Mensajero
/
Ratas Wistar
/
Receptores Citoplasmáticos y Nucleares
/
Biología Celular
/
Hígado
/
Cirrosis Hepática
Límite:
Animales
Idioma:
Chino
Revista:
Chinese Journal of Hepatology
Año:
2004
Tipo del documento:
Artículo