Determination of 5-hydroxy-7-methoxyflavan in Daemonorops draco by HPLC / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To establish a method for the determination of 5-hydroxy-7-methoxyflavan in Daemrnonorops draco by HPLC.</p><p><b>METHOD</b>Dikma Diamonsil C15 column(4. 6 mm x 150 mm, 5 microm) was used. The mobile phase consisted of (6040). The temperature of column was 30 degrees C. The flow rate was 10 mL x min(-1) and the detection wavelength was at 210 nm.</p><p><b>RESULT</b>The calibration curve was in good linearity with in the range from 0.01 to 0.10 microg (r = 0.9999). The average recovery was 100.1% (n=6).</p><p><b>CONCLUSION</b>The method is simple, rapid, as well as precise and reliable, and can be used for the determination of 5-hydroxy-7-methoxyflavan in D. draco.</p>