Omega-6 polyunsaturated fatty acid promotes colon carcinogenesis / 中华胃肠外科杂志
Chinese Journal of Gastrointestinal Surgery
; (12): 774-777, 2010.
Article
en Zh
| WPRIM
| ID: wpr-266271
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate whether ω-6 polyunsaturated fatty acid promotes colon carcinogenesis through downregulation of P53-dependent growth inhibition.</p><p><b>METHODS</b>Colon carcinogenesis was induced by injection of azoxymethane (AOM) intraperitoneally. Experimental animals were randomly divided into four groups, receiving regular diet and intraperitoneal injection of normal saline(control group), high ω-6 polyunsaturated fatty acid diet with intraperitoneal injection of normal saline(Corn oil group), regular diet with intraperitoneal injection of AOM(AOM group), or high ω-6 polyunsaturated fatty acid diet with intraperitoneal injection of AOM (Corn oil+AOM group). Aberrant crypt focis (ACFs) were observed after methylene blue staining and enumerated. Colonic mucosa PCNA and P53 expressions were assessed by RT-PCR and Western blotting, and location of P53 in the colon crypt focis was determined by immunohistochemical staining.</p><p><b>RESULTS</b>Amounts of ACFs was 1.2±0.3 in the control group, 1.3±0.4 in the Corn oil group, 41.0±4.8 in the AOM group, and 73.3±9.9 in the Corn oil+AOM group, the differences were statistically significant(P<0.05). The expression of P53 in normal crypt focis was higher than that in ACFs. High ω-6 polyunsaturated fatty acid dietary significantly promoted AOM-induced colon PCNA expression, and enhanced AOM-mediated P53 inhibition in colon mucosa.</p><p><b>CONCLUSION</b>High ω-6 polyunsaturated fatty acid diet can enhance AOM-induced inhibition of P53 in colon mucosa, resulting in overexpression of PCNA, formation of ACF, and carcinogenesis in the colon.</p>
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Proteína p53 Supresora de Tumor
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Ratas Sprague-Dawley
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Colon
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Antígeno Nuclear de Célula en Proliferación
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Ácidos Grasos Insaturados
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Mucosa Intestinal
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Metabolismo
Límite:
Animals
Idioma:
Zh
Revista:
Chinese Journal of Gastrointestinal Surgery
Año:
2010
Tipo del documento:
Article