Effect of PDK1 on Neutrophil Differentiation / 中国实验血液学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To knock out PDK1 by using Vav-Cre and observe the effects of PDK1 knock out on the ratio, number and differentiation of neutrophil.</p><p><b>METHODS</b>The PDK1 expression level of Vav-Cre;PDK1mouse in the bone marrow cells was analyzed by RT-PCR. The effect of PDK1 on hematopoietic progenitor was observed by CFU-C assay, and the effect of PDK1 on the ratio and number of neutrophil was detected by flow cytometric analysis.</p><p><b>RESULTS</b>The RNA expression level of bone marrow cells of Vav-Cre;PDK1mouse was dramatically lower than that of the control mouse. The number of functional GMP was lower in Vav-Cre;PDK1mouse in contrast to controls. The percentage and number of neutrophil were lower, but the percentage of premyelocyte/myelocytes was more than 2 times in Vav-Cre;PDK1group compared with control groups.</p><p><b>CONCLUSION</b>PDK1 affects the process of the differention of hematopoietic stem cells to GMP, the neutrophil differention and maturation.</p>