Research on the effects of PIAS3 expression on the invasion of glioma TJ905 cells / 中华外科杂志
Chinese Journal of Surgery
; (12): 440-444, 2011.
Artículo
en Chino
| WPRIM (Pacífico Occidental)
| ID: wpr-285705
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVES</b>To investigate the function and possible mechanisms of PIAS3 expression on the invasion of TJ905 cells.</p><p><b>METHODS</b>PIAS3 overexpression vectors were constructed and PIAS3 siRNA were chemically synthesized, which were separately transfected into TJ905 cells for upregulation or downregulation of PIAS3 expression levels in TJ905 cells. After that, the invasive effects of TJ905 cells were measured by Transwell assay, and the expression of PIAS3, tissue inhibitor of metalloproteinases (TIMP)3, matrix metalloprotease (MMP)-2, and MMP-9 were identified by Western blot.</p><p><b>RESULTS</b>In vitro transfection efficiency of plasmids and oligonucleotides were separately 85.3% ± 3.1% and 95.1% ± 2.9%. PIAS3 overexpression plasmid transfection in vitro could effectively improve the expression of PIAS3 protein in TJ905 cells and inhibit the invasion of TJ905 cells (P < 0.05), and cell penetration ratio reduced from 87.9% ± 9.3% to 37.3% ± 7.9% compared with control group, while it upregulated TIMP3 and downregulated MMP-2, MMP-9 protein expression (P < 0.05); PIAS3 siRNA transfection could inhibit the PIAS3 protein expression of TJ905 cells and promote the invasion of TJ905 cells (P < 0.05), and cell penetration ratio increased from 83.9% ± 7.1% to 93.2% ± 3.1% compared with control group, while it downregulated TIMP3 and upregulated MMP-2, MMP-9 protein expression (P < 0.05).</p><p><b>CONCLUSION</b>PIAS3 expression is closely related to the invasion properties of glioma TJ905 cells.</p>
Texto completo:
Disponible
Base de datos:
WPRIM (Pacífico Occidental)
Asunto principal:
Patología
/
Transfección
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Chaperonas Moleculares
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Inhibidor Tisular de Metaloproteinasa-3
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Metaloproteinasa 2 de la Matriz
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Metaloproteinasa 9 de la Matriz
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ARN Interferente Pequeño
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Línea Celular Tumoral
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Proteínas Inhibidoras de STAT Activados
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Vectores Genéticos
Límite:
Humanos
Idioma:
Chino
Revista:
Chinese Journal of Surgery
Año:
2011
Tipo del documento:
Artículo