Antiglioma activity of endothelial progenitor cells transduced with HSV-TK via inhibiting angiogenesis in vitro and in vivo / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
; (6): 170-174, 2009.
Artículo
en Chino
| WPRIM (Pacífico Occidental)
| ID: wpr-287431
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the potentiality of herpes simplex virus thymidine kinase transduced endothelial progenitor cells (EPC-TK) as angiogenesis-targeting vector in the glioma treatment in vitro and in vivo.</p><p><b>METHODS</b>EPC-TK were mixed with human umbilical vein endothelial cells (HUVECs), U87 or U251 cells at various ratios for ganciclovir (GCV) treatment. The bystander effect was observed by counting the survival cells using MTT assay, and the apoptotic cells were determined by annexin-V and propidium iodide (PI) staining. EPC-TK, EPCs, or phosphate buffered saline (PBS) were injected into the nude mice model of glioma xenograft by tail vein, for the EPC-TK group, EPC group, and PBS group, respectively. And then the changes of tumor volume and tumor vasculature were observed.</p><p><b>RESULTS</b>GCV killed most EPC-TK and reduced the number of other viable cells in a cellcell ratio-dependent and time-dependent manner. EPC-TK obviously inhibited tumor growth. The tumor volumes on day 21 were 1741.20+/- 576.10 mm(3), 3275.52 +/- 710.86 mm(3) and 3033.09+/-1134.86 mm(3) in the EPC-TK, EPC and PBS group, respectively. EPC-TK also displayed a significant effect on the inhibition of tumor angiogenesis.</p><p><b>CONCLUSION</b>EPC-TK can exert a potent antiglioma effect via inhibiting angiogenesis.</p>
Texto completo:
Disponible
Base de datos:
WPRIM (Pacífico Occidental)
Asunto principal:
Antivirales
/
Farmacología
/
Fisiología
/
Terapéutica
/
Timidina Quinasa
/
Transducción Genética
/
Virología
/
Transfección
/
Transformación Celular Viral
/
Simplexvirus
Tipo de estudio:
Estudio pronóstico
Límite:
Animales
/
Humanos
Idioma:
Chino
Revista:
Chinese Journal of Medical Genetics
Año:
2009
Tipo del documento:
Artículo