Establishment of indirect immunofluorescence assay (IFA) for detection of IgG antibody against new bunyavirus / 中华预防医学杂志
Chinese Journal of Preventive Medicine
; (12): 165-168, 2012.
Artículo
en Chino
| WPRIM (Pacífico Occidental)
| ID: wpr-292501
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To develop an indirect immunofluorescence assay (IFA) for detection of IgG antibodies against new bunyavirus.</p><p><b>METHODS</b>The antigen slides were prepared with 5 new bunyavirus strains isolated using Africa green monkey kidney (Vero) cells. Specificity and sensitivity evaluation of IFA were carried out by optimizing working conditions of IFA. Using established IFA, serum samples from both acute and recovery phases were tested for 126 cases with fever thrombocytopenia and leukopenia syndrome in Xinyang, Henan province in 2007 - 2011. The results were compared with detections by RT-PCR.</p><p><b>RESULTS</b>The new bunyavirus stable immunofluorescence specific WZ69 strain was selected to prepare antigen slides of IFA. The optimum conditions of IFA were optimum dilution for primary antibody (serum) and secondary antibody (isosulfocyanic acid fluorescence marked goat anti-human IgG antibody) was 140 and 1150 respectively. The optimum dilution for Evans blue in secondary antibody was 120 000. Among the 126 patients, 96 paired serum specimens were tested positive to the new bunyavirus and 30 patients were tested negative to the virus. The positive rate of antibodies was 76.19%. There was no significant difference in results between IFA and RT-PCR (72.22% (91/126)) (P > 0.05).</p><p><b>CONCLUSION</b>The IFA has high sensitivity and specificity with easy operation. It can be used in detecting the new bunyavirus infection in patients with fever, thrombocytopenia and leukopenia syndrome.</p>
Texto completo:
Disponible
Base de datos:
WPRIM (Pacífico Occidental)
Asunto principal:
Células Vero
/
Inmunoglobulina G
/
Chlorocebus aethiops
/
Sensibilidad y Especificidad
/
Orthobunyavirus
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Infecciones por Bunyaviridae
/
Técnica del Anticuerpo Fluorescente Indirecta
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Diagnóstico
/
Alergia e Inmunología
/
Anticuerpos Antivirales
Tipo de estudio:
Estudio diagnóstico
Límite:
Animales
/
Femenino
/
Humanos
/
Masculino
Idioma:
Chino
Revista:
Chinese Journal of Preventive Medicine
Año:
2012
Tipo del documento:
Artículo