Identification of a novel PHOX2A gene mutation in a Chinese family with congenital fibrosis of extraocular muscles type 2 / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
; (6): 5-8, 2012.
Article
en Zh
| WPRIM
| ID: wpr-295545
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate potential mutation of PHOX2A (or ARIX) gene in a Chinese family affected with congenital fibrosis of extraocular muscles tyep 2 (CFEOM2).</p><p><b>METHODS</b>Genomic DNA was obtained from affected and unaffected members of the family. With an ABI PRSIM Linkage Mapping Set-MD10 kit, selected markers flanking the PHOX2A locus were used for linkage analysis. Exons of PHOX2 gene were amplified and sequenced. A total of 100 normal subjects were recruited as controls.</p><p><b>RESULTS</b>Genetic linkage was found at 11q13 between D11S4151 and D11S1320 and the PHOX2A gene. DNA sequencing has identified a heterozygous mutation in the exon 2 of the gene (227T to G, N76K). The same mutation was not found in the unaffected and 100 normal controls.</p><p><b>CONCLUSION</b>A mutation of the PHOX2A gene 227T to G is responsible for the onset of congenital fibrosis of extraocular muscles type 2 in this Chinese family.</p>
Texto completo:
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Base de datos:
WPRIM
Asunto principal:
Linaje
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Anomalías Congénitas
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Fibrosis
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Datos de Secuencia Molecular
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Secuencia de Bases
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Estudios de Casos y Controles
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Trastornos de la Motilidad Ocular
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China
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Proteínas de Homeodominio
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Genética
Tipo de estudio:
Observational_studies
Límite:
Female
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Humans
/
Male
País/Región como asunto:
Asia
Idioma:
Zh
Revista:
Chinese Journal of Medical Genetics
Año:
2012
Tipo del documento:
Article