Research on bioactive ingredients in rat liver after oral administration of different combinations of Wuji pill / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 1695-1703, 2014.
Artículo
en Chino
| WPRIM (Pacífico Occidental)
| ID: wpr-300203
Biblioteca responsable:
WPRO
ABSTRACT
A L9 (3(4)) orthogonal design table to be used to get nine combinations of extraction of three herbs of Wuji pill Coptis chinensis, Tetradium ruticarpum and Paeonia lactiflora Pall., and nine extraction of single herbs correspondingly, altogether eighteen combinations. Quantification of five representative bioactive ingredients berberine, palmatine, evodiamine, rutaecarpine, paeoniflorin in rat liver by ultra high liquid chromatography-tandem mass spectrometry after oral administration at 2 h time point of eighteen combinations. The result shows the bioactive ingredients have different concentrations betweem different combinations and the single herb with the same dosage significantly as well as the same dose combinations. C. chinensis with evodiamine concentration of low and high dose T. ruticarpum was positively correlated. T. ruticarpum with berberine concentration of low dose C. chinensis was negatively correlated and of meddle dose C. chinensis was correlated positively. T. ruticarpum with paeoniflorin concentration of middle dose P. lactiflora was correlated positively. P. lactiflora with palmatine concentration of middle dose C. chinensis was negatively correlated and with evodiamine and rutaecarpine concentration of middle dose T. ruticarpum was negatively correlated. These shows the three single herbs interactions resulted in the differences of each ingredients concentration in rat liver. The orthogonal analysis indicates the combination 12 6 6 make the maximum concentration in rat liver.
Texto completo:
Disponible
Base de datos:
WPRIM (Pacífico Occidental)
Asunto principal:
Plantas Medicinales
/
Temperatura
/
Medicamentos Herbarios Chinos
/
Farmacocinética
/
Disponibilidad Biológica
/
Química
/
Administración Oral
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Cromatografía Líquida de Alta Presión
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Ratas Sprague-Dawley
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Investigación Biomédica
Límite:
Animales
Idioma:
Chino
Revista:
China Journal of Chinese Materia Medica
Año:
2014
Tipo del documento:
Artículo