Gene copy number, mRNA transcription and protein expression of PD-1 gene in primary hepatocarcinoma patients / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
; (6): 282-284, 2012.
Article
en Zh
| WPRIM
| ID: wpr-305058
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the gene copy number, mRNA transcription and protien expression of programmed cell death 1 (PD-1) gene in primary hepatocellular carcinoma (PHC) patients and normal control individuals (NC) who are anti-HBs positive, and to investigate the variations in PD-1 gene copy numbers and its relationship with PHC.</p><p><b>METHODS</b>Real-time PCR was adopted to detect the PD-1 gene copy numbers and their mRNA expressions in peripheral blood mononuclear cells (PBMCs) from 24 samples of PHC patients and 26 of NC. Protein expression level of PD-1 on CD8+ T was analyzed by flow cytometry.</p><p><b>RESULTS</b>In terms of number of PD-1 gene copy numbers, the percentage of cases of haploid (single) was 34.62% and 4.17% in PHC group and control group respectively while the percentage of cases of diploid (double) was 61.54% and 95.83% respectively. The difference between the two was statistically significant (chi2 = 7.639, P = 0.006). The rate of cases with double PD-1 gene copy numbers was found to be higher in patients with PHC than in control group. It was also found that the average expression of PD-1 mRNA was 2.35E-03 in control group and 1.23E-03 in PHC group. The expression level was significant lower in PHC group than that in control group when compared by using Mann-whitey technic (U = 153, P = 0.009). Furthermore, the frequency of PD-1 protein expression on CD8+ T cells was 3.72 +/- 0.32 in control group and 16.13 +/- 1.68 in PHC group. The level of PD-1 mRNA expression was higher in PHC and significant differences was shown between two groups (t = -7.073, P = 0.000).</p><p><b>CONCLUSIONS</b>Our study suggests that the variation in PD-1 gene copy number may trigger primary hepatocellular carcinoma to HBV carriers. The relationship between the variation of PD-1 gene copy numbers and its association with primary hepatocellular carcinoma is worth further focus.</p>
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Transcripción Genética
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ARN Mensajero
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Estudios de Casos y Controles
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Regulación Neoplásica de la Expresión Génica
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Carcinoma Hepatocelular
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Dosificación de Gen
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Receptor de Muerte Celular Programada 1
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Genética
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Neoplasias Hepáticas
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Metabolismo
Tipo de estudio:
Observational_studies
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Risk_factors_studies
Límite:
Adult
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Female
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Humans
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Male
Idioma:
Zh
Revista:
Chinese Journal of Experimental and Clinical Virology
Año:
2012
Tipo del documento:
Article