First Report in China on the Identification and Drug Sensitivity of Mycobacterium elephantis Isolated from the Milk of a Cow with Mastitis / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
; (12): 501-507, 2017.
Artículo
en Inglés
| WPRIM (Pacífico Occidental)
| ID: wpr-311386
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>In this study, milk from a cow with mastitis was analyzed to determine the presence of mycobacterial infection. Milk quality and security problems pertaining to the safe consumption of dairy products were also discussed in this study.</p><p><b>METHODS</b>Milk was preprocessed with 4% NaOH. Then, mycobacteria were isolated from the milk sample on L-J medium. The isolate was identified using multiple loci Polymerase Chain Reaction (PCR) and multi-locus sequence analysis with 16S rRNA, sodA, hsp65, and ITS genes. The drug sensitivity of the isolate to 27 antibiotics was tested through alamar blue assay.</p><p><b>RESULTS</b>Smooth, moist, pale yellow colonies appeared on the L-J medium within a week after inoculation. Based on the results of multiple loci PCR analysis, the isolate was preliminarily identified as non-tuberculous mycobacteria. The 16S rRNA, SodA, hsp65, and ITS gene sequences of the isolate exhibited 99%, 99%, 99%, and 100% similarities, respectively, with those of the published reference strains of Mycobacterium elephantis (M. elephantis). The drug sensitivity results showed that the strain is resistant to isoniazid, p-aminosalicylic acid, and trimesulf but is sensitive to ofloxacin, rifampicin, amikacin, capreomycin, moxifloxacin, kanamycin, levofloxacin, cycloserine, ethambutol, streptomycin, tobramycin, rifabutin, ciprofloxacin, linezolid, cefoxitin, clarithromycin, and minocycline.</p><p><b>CONCLUSION</b>To the best of our knowledge, this study is initially to report the isolation of M. elephantis from the milk of a cow with mastitis in China.</p>
Texto completo:
Disponible
Contexto en salud:
Enfermedades Desatendidas
Problema de salud:
Zoonosis
Base de datos:
WPRIM (Pacífico Occidental)
Asunto principal:
Farmacología
/
Filogenia
/
China
/
Reacción en Cadena de la Polimerasa
/
Epidemiología
/
Farmacorresistencia Bacteriana
/
Leche
/
Genética
/
Mastitis Bovina
/
Microbiología
Tipo de estudio:
Estudio diagnóstico
Límite:
Animales
País/Región como asunto:
Asia
Idioma:
Inglés
Revista:
Biomedical and Environmental Sciences
Año:
2017
Tipo del documento:
Artículo