Changes in mRNA expression of p53 and related downstream genes in peripheral blood lymphocytes in workers occupationally exposed to arsenic / 中华劳动卫生职业病杂志
Chinese Journal of Industrial Hygiene and Occupational Diseases
; (12): 891-893, 2015.
Artículo
en Chino
| WPRIM (Pacífico Occidental)
| ID: wpr-312096
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the changes in mRNA expression of p53 and related downstream genes in peripheral blood lymphocytes in workers occupationally exposed to arsenic as well as its influencing factors, and to analyze the mechanism of genetic toxicity of arsenic.</p><p><b>METHODS</b>With cluster random sampling, 79 workers from an arsenic smelting plant were selected as exposure group, and another 24 people without occupational exposure to arsenic were selected as control group. The relative mRNA expression of p53 and related downstream genes in the peripheral blood lymphocytes of the two groups was determined by quantitative realtime PCR. The levels of inorganic arsenic (iAs), monomethylarsonic acid (MMA), and dimethylarsinic acid (DMA) in urine were determined by hydride generation-atomic absorption spectrometry.</p><p><b>RESULTS</b>The exposure group had significantly higher levels of iAs, MMA, and DMA than the control group (P<0.01); the exposure group had significantly higher relative mRNA expression (2(-ΔΔCt)) of p53 and four related downstream genes in peripheral blood lymphocytes than the control group (P<0.05); the relative mRNA expression of p53 and related downstream genes was positively correlated with each other (P<0.01), with a correlation coefficient greater than 0.4; the levels of arsenic compounds in urine were positively correlated with the relative mRNA expression of p53 and some of its downstream genes (P<0.05).</p><p><b>CONCLUSION</b>The changes in mRNA expression of p53 and related downstream genes are closely related to the metabolic transformation of inorganic arsenic in workers occupationally exposed to arsenic, and it also plays an important role in genetic toxicity and carcinogenic effect in people exposed to arsenic.</p>
Texto completo:
Disponible
Contexto en salud:
ODS3 - Meta 3.9 Reducir las muertes producidas por productos químicos peligrosos y la contaminación del aire, el agua y el suelo
Problema de salud:
Exposiciones Ocupacionales
Base de datos:
WPRIM (Pacífico Occidental)
Asunto principal:
Arsénico
/
Arsenicales
/
Orina
/
Ácido Cacodílico
/
ARN Mensajero
/
Linfocitos
/
Estudios de Casos y Controles
/
Exposición Profesional
/
Proteína p53 Supresora de Tumor
/
Metabolismo
Tipo de estudio:
Estudio observacional
Límite:
Humanos
Idioma:
Chino
Revista:
Chinese Journal of Industrial Hygiene and Occupational Diseases
Año:
2015
Tipo del documento:
Artículo