Expression of TGF-beta1/Smad protein in rat liver fibrosis model and the role of IFN-gamma / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
; (6): 340-343, 2013.
Artículo
en Chino
| WPRIM (Pacífico Occidental)
| ID: wpr-318026
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the impact of IFN-gamma on liver fibrosis and its possible mechanism. Thirty healthy male SD rats were randomly divided into two groups fibrosis model group, IFN-gamma treatment group. Experimental liver fibrosis was induced by subcutaneous injection of CCl4. After 12-week-treatment, serum hyalurnic acid and TGF-beta1 was examined, histopathological changes and degrees of fibrosis were observed by optical microscopy. Meanwhile, the expression of TGF-beta1, TbetaR- I and Smad2/3 proteins was detected by immunohistochemistry and quantified by using computerized image analysis.</p><p><b>RESULTS</b>(1) Pathological observation of hepatic specimens histological examination showed that there were significant difference between normal group and fibrosis model group by comparing with the degrees of inflammation and fibrosis (P < 0.05). And the difference between fibrosis model group and IFN-gamma treatment group was significant (P < 0.05). (2) Changes of the hepatic fibrosis index (serum HA and TGF-beta1) the levels of serum HA, TGF-beta1 in fibrosis model group were higher than IFN-gamma treatment groups (P < 0.05). (3) Changes of gene protein levels about TGF-beta1/Smad the expressions of TGF-beta1, TbetaR- I and Smad2/3 in rat hepatic tissue were detected with immunohistochemistry techniques. The expressions of the three items in model group were higher than normal group (P < 0.01). The difference between model group and IFN-gamma treatment group was significant (P < 0.05);</p><p><b>CONCLUSION</b>IFN-gamma treatment group had significant results on treating experimental hepatic fibrosis. By the way of inhibiting expressions of TGF-beta1, TbetaR- I, Smad2/3, IFN-gamma treatment group exerted its anti-fibrosis effect.</p>
Texto completo:
Disponible
Base de datos:
WPRIM (Pacífico Occidental)
Asunto principal:
Interferón gamma
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Ratas Sprague-Dawley
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Usos Terapéuticos
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Modelos Animales de Enfermedad
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Quimioterapia
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Proteína Smad2
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Proteína smad3
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Factor de Crecimiento Transformador beta1
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Genética
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Hígado
Tipo de estudio:
Estudio pronóstico
Límite:
Animales
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Humanos
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Masculino
Idioma:
Chino
Revista:
Chinese Journal of Experimental and Clinical Virology
Año:
2013
Tipo del documento:
Artículo