The effect of protein kinase B on the expression and location of p21 in early development of mouse fertilized eggs / 生物工程学报
Chinese Journal of Biotechnology
; (12): 493-496, 2007.
Article
en Zh
| WPRIM
| ID: wpr-327998
Biblioteca responsable:
WPRO
ABSTRACT
To investigate the effect of Protein kinase B on the expression and location of p21 in mouse early development. Immunopreciptation technology was used to detect the localization of p21 and Western blotting was used to analyze the expression of p21 after microinjecting mRNA of WT-PKB, myt-PKB and PKB-KD to mouse eggs. There was no obvious difference between the three kinds of mRNA in the expression of p21. But the cell localization altered. The p21 retain in cytoplasm after microjecting myt-PKB. In mouse fertilized egg PKB/Akt controls the cell cycle by changing the cell localization of p21.
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Factores de Tiempo
/
Cigoto
/
ARN Mensajero
/
Núcleo Celular
/
Técnica del Anticuerpo Fluorescente
/
Biología Celular
/
Citoplasma
/
Proteínas Proto-Oncogénicas c-akt
/
Inhibidor p21 de las Quinasas Dependientes de la Ciclina
/
Genética
Límite:
Animals
Idioma:
Zh
Revista:
Chinese Journal of Biotechnology
Año:
2007
Tipo del documento:
Article