Cytogenetic and molecular analysis of 1193 cases with chronic myeloid leukemia / 中华血液学杂志
Chinese Journal of Hematology
; (12): 1-5, 2007.
Article
en Zh
| WPRIM
| ID: wpr-328383
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the cytogenetic and molecular genetic features of chronic myeloid leukemia (CML) in Chinese.</p><p><b>METHODS</b>A total of 1193 CML patients were retrospectively studied. Chromosome preparation of bone marrow cells was made using direct and short-term culture. Karyotype and bcr-abl fusion genes were analyzed by R-banding, RT-PCR, respectively.</p><p><b>RESULTS</b>In the 1193 cases, 98.07% was Ph chromosome positive (Ph+) and 1.93% negative (Ph-). In the Ph+ patients, 95.64% was classical Ph and 4.36% variant rearrangements. Additional genetic changes were demonstrated in 11.88% of classical Ph cases. Cytogenetic clonal evolution was found in 7.94% of patients in chronic phase (CP), 27.78% in accelerated phase (AP), and 49. 04% in blast crisis (BC). Among the classical Ph cases, +Ph, +8, -21 were found in 14.62%, 10.77% and 7.69% of them respectively. In patients in BC and AP, the most common additional chromosome changes were + Ph (28.57%), +8 (16.67%) and +19 (7.14%), while in CP, -21 (10.26%), +Ph (8.97%), and +8 (8.97%). The combination of +Ph and +8 (3.60%) was the most frequent of combination pattern. 524 cases were investigated for bcr-abl fusion gene, and 54.01% was b3a2 (+) and 27.67% b2a2 (+).</p><p><b>CONCLUSION</b>In Chinese CML patients seem to have their unique features in terms of cytogenetic clonal evaluation.</p>
Texto completo:
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Base de datos:
WPRIM
Asunto principal:
Leucemia Mielógena Crónica BCR-ABL Positiva
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Estudios Retrospectivos
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Proteínas de Fusión bcr-abl
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Genética
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Cariotipificación
Tipo de estudio:
Observational_studies
Límite:
Adolescent
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Adult
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Aged
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Child
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Child, preschool
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Female
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Humans
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Male
Idioma:
Zh
Revista:
Chinese Journal of Hematology
Año:
2007
Tipo del documento:
Article