Regulation mechanism for rig-g gene expression induced by all-trans retinoic acid / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 31-35, 2010.
Artículo
en Chino
| WPRIM (Pacífico Occidental)
| ID: wpr-328578
Biblioteca responsable:
WPRO
ABSTRACT
To investigate the molecular mechanisms of all-trans retinoic acid (ATRA)-induced rig-g gene expression and to better understand the signal transduction of ATRA during acute promyelocytic leukemia (APL) cell differentiation, the luciferase reporter assay, co-immunoprecipitation and chromatin immunoprecipitation were used to clarify the basic transcriptional factors, which directly initiated the expression of rig-g gene. The results showed that the expression of STAT2, IRF-9 and IRF-1 could be upregulated by ATRA with different kinetics in NB4 cells. IRF-9 was able to interact with STAT2 to form a complex, which could bind the rig-g gene promoter and trigger the rig-g expression. IRF-1 alone could also activate the reporter gene containing rig-g gene promoter, but C/EBPalpha could strongly inhibit this transcription activity of IRF-1. It is concluded that during ATRA-induced APL cell differentiation, IRF-1 is first upregulated by ATRA, and then IRF-1 increases the protein levels of IRF-9 and STAT2 with the downregulation of C/EBPalpha. The complex of IRF-9 and STAT2 is the primary transcriptional factor for rig-g gene induction. This study will be helpful for better understanding the signal transduction networks of ATRA during the course of APL cell differentiation.
Texto completo:
Disponible
Base de datos:
WPRIM (Pacífico Occidental)
Asunto principal:
Farmacología
/
Tretinoina
/
Células Tumorales Cultivadas
/
Leucemia Promielocítica Aguda
/
Transducción de Señal
/
Regulación Leucémica de la Expresión Génica
/
Genes Reguladores
/
Proteína alfa Potenciadora de Unión a CCAAT
/
Péptidos y Proteínas de Señalización Intracelular
/
Factor de Transcripción STAT2
Límite:
Humanos
Idioma:
Chino
Revista:
Journal of Experimental Hematology
Año:
2010
Tipo del documento:
Artículo