Ampelopsin, a small molecule inhibitor of HIV-1 infection targeting HIV entry / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
; (12): 153-164, 2004.
Artículo
en Inglés
| WPRIM (Pacífico Occidental)
| ID: wpr-329646
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the anti-HIV effects of ampelopsin and its interaction with HIV-1 coreceptor CXCR4.</p><p><b>METHODS</b>Through anti-virus experiments in vitro, the inhibitory effect of ampelopsin on HIV-1 infection was verified. Chemotaxis assay was performed to show the ability to induce PBMCs migration by ampelopsin, RANTES and SDF-1alpha. Fluorescence labelling monoclonal antibody was utilized to observe the interaction of ampelopsin and CXCR4. Mice immunosuppressant model was also established to detail the role ampelopsin played in regulating cellular immunological functions.</p><p><b>RESULTS</b>Ampelopsin could protect sensitive cells against HIV-1 infection and dramatically reduce HIV-1 antigen P24 expression. HIV-1SF33 attaching to MT-4 cells was interfered by ampelopsin, and the EC50 was 0.175 mg/mL for cellular protection and 0.024 mg/mL for P24 inhibition. At co-cultivating phase, EC50 was 0.229 mg/mL and 0.197 mg/mL respectively. Furthermore, the EC50 was 0.179 mg/mL and 0.348 mg/mL in acute infection. Human PBMCs migration was induced after being challenged with ampelopsin or chemokines, and synergistic action was observed during co-treatment. Ampelopsin alone resulted in maximal chemotaxis at 1 mg/mL. HIV-1 co-receptor CXCR4 on the surface of PBMCs was decreased by internalization, which indicated the effect of ampelopsin on CXCR4. About 70% CXCR4 was reduced by ampelopsin at 1 mg/mL. Ampelopsin also augmented cellular immunological functions in immunosuppressive mice.</p><p><b>CONCLUSION</b>Ampelopsin displays a strong inhibitive role during HIV-1 absorption, incubation and acute infection. These results are coincident with its immune enhancement.</p>
Texto completo:
Disponible
Contexto en salud:
ODS3 - Salud y Bienestar
Problema de salud:
Meta 3.3: Poner fin a las enfermedades desatendidas y detener enfermedades transmisibles
Base de datos:
WPRIM (Pacífico Occidental)
Asunto principal:
Farmacología
/
Bazo
/
Virología
/
Virulencia
/
Flavonoides
/
Medicamentos Herbarios Chinos
/
Leucocitos Mononucleares
/
Linfocitos T
/
Regulación hacia Abajo
/
Infecciones por VIH
Tipo de estudio:
Evaluación económica en salud
/
Estudio pronóstico
Aspecto:
Determinantes sociales de la salud
Límite:
Animales
/
Humanos
Idioma:
Inglés
Revista:
Biomedical and Environmental Sciences
Año:
2004
Tipo del documento:
Artículo