Breeding of transgenic mice expressing human tau isoform with P301L mutation and identification of homozygous transgenic mice / 中国应用生理学杂志
Chinese Journal of Applied Physiology
; (6): 221-224, 2012.
Article
en Zh
| WPRIM
| ID: wpr-329903
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To establish homozygous transgenic mouse strain expressing human tau isoform with P301L mutation.</p><p><b>METHODS</b>Five transgenic mice expressing human tau isoform with P301L mutation were obtained by microinjection into male nuclei. Homozygote and hemizygote were identified by PCR and real-time fluorescent quantitative PCR.</p><p><b>RESULTS</b>Ninety five homozygous transgenic mice were selected, and the results indicated that homozygous transgenic mice were superior to hemizygote in simulating the changes of biological characteristics.</p><p><b>CONCLUSION</b>Exogenous gene tau is able to stably transmit to next generation and the combination of SYBR Green real-time fluorescent quantitative PCR with the traditional mating is a fast, reliable and economical way to screen homozygous and hemizygous transgenic mice.</p>
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Ratones Transgénicos
/
Proteínas tau
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Genética
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Homocigoto
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Ratones Endogámicos C57BL
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Microinyecciones
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Mutación
Tipo de estudio:
Diagnostic_studies
/
Prognostic_studies
Límite:
Animals
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Female
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Humans
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Male
Idioma:
Zh
Revista:
Chinese Journal of Applied Physiology
Año:
2012
Tipo del documento:
Article